Our Publications
2014
101.
Peyachoknagul, S.; Mongkolsiriwatan, C.; Wannapinpong, S.; Huehne, P. S.; Srikulnath, K.
Identification of native Dendrobium species in Thailand by PCR-RFLP of rDNA-ITS and chloroplast DNA Journal Article
In: ScienceAsia, vol. 40, no. 2, pp. 113-120, 2014, ISSN: 15131874, (cited By 10).
@article{Peyachoknagul2014113,
title = {Identification of native Dendrobium species in Thailand by PCR-RFLP of rDNA-ITS and chloroplast DNA},
author = {S. Peyachoknagul and C. Mongkolsiriwatan and S. Wannapinpong and P. S. Huehne and K. Srikulnath},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84902174123&doi=10.2306%2fscienceasia1513-1874.2014.40.113&partnerID=40&md5=40f8408482fef3f1deb0581f913ebe3f},
doi = {10.2306/scienceasia1513-1874.2014.40.113},
issn = {15131874},
year = {2014},
date = {2014-01-01},
journal = {ScienceAsia},
volume = {40},
number = {2},
pages = {113-120},
publisher = {Science Society of Thailand under Royal Patronage},
abstract = {The PCR-restriction fragment length polymorphism (PCR-RFLP) approach was successfully developed to identify 25 native Dendrobium species in Thailand. PCR-RFLP of the rDNA-ITS with six restriction enzymes and three chloroplast (cp) DNA regions with five primer-enzyme combinations produced 24 types of DNA patterns altogether. Twenty-three out of the 25 species determined in this study were found to belong to unique classes and were successfully differentiated. Two species, D. crumenatum and D. formosum, possessing the same DNA pattern, however, were identified after cutting the chloroplast DNA fragment amplified by psbC-trnS primer with MboI enzyme. An effective procedure for identifying each Dendrobium species was developed. PCR-RFLP of the rDNA-ITS with TaqI, which is the most informative enzyme, was used for the early detection of 16 Dendrobium species. To identify the remaining Dendrobium species, PCRRFLP analysis was performed using one more primer-enzyme combination. Our study provides a rapid, simple, and reliable identification method for these Dendrobium species.},
note = {cited By 10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The PCR-restriction fragment length polymorphism (PCR-RFLP) approach was successfully developed to identify 25 native Dendrobium species in Thailand. PCR-RFLP of the rDNA-ITS with six restriction enzymes and three chloroplast (cp) DNA regions with five primer-enzyme combinations produced 24 types of DNA patterns altogether. Twenty-three out of the 25 species determined in this study were found to belong to unique classes and were successfully differentiated. Two species, D. crumenatum and D. formosum, possessing the same DNA pattern, however, were identified after cutting the chloroplast DNA fragment amplified by psbC-trnS primer with MboI enzyme. An effective procedure for identifying each Dendrobium species was developed. PCR-RFLP of the rDNA-ITS with TaqI, which is the most informative enzyme, was used for the early detection of 16 Dendrobium species. To identify the remaining Dendrobium species, PCRRFLP analysis was performed using one more primer-enzyme combination. Our study provides a rapid, simple, and reliable identification method for these Dendrobium species.
2013
102.
Srikulnath, K.; Uno, Y.; Nishida, C.; Matsuda, Y.
Karyotype evolution in monitor lizards: Cross-species chromosome mapping of cDNA reveals highly conserved synteny and gene order in the Toxicofera clade Journal Article
In: Chromosome Research, vol. 21, no. 8, pp. 805-819, 2013, ISSN: 09673849, (cited By 43).
@article{Srikulnath2013805,
title = {Karyotype evolution in monitor lizards: Cross-species chromosome mapping of cDNA reveals highly conserved synteny and gene order in the Toxicofera clade},
author = {K. Srikulnath and Y. Uno and C. Nishida and Y. Matsuda},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891836648&doi=10.1007%2fs10577-013-9398-0&partnerID=40&md5=b758ce5e7424dd23295ad1feff5bcb19},
doi = {10.1007/s10577-013-9398-0},
issn = {09673849},
year = {2013},
date = {2013-01-01},
journal = {Chromosome Research},
volume = {21},
number = {8},
pages = {805-819},
abstract = {The water monitor lizard (Varanus salvator macromaculatus (VSA), Platynota) has a chromosome number of 2n = 40: its karyotype consists of 16 macrochromosomes and 24 microchromosomes. To delineate the process of karyotype evolution in V. salvator macromaculatus, we constructed a cytogenetic map with 86 functional genes and compared it with those of the butterfly lizard (Leiolepis reevesii rubritaeniata (LRE); 2n = 36) and Japanese four-striped rat snake (Elaphe quadrivirgata (EQU); 2n = 36), members of the Toxicofera clade. The syntenies and gene orders of macrochromosomes were highly conserved between these species except for several chromosomal rearrangements: eight pairs of VSA macrochromosomes and/or chromosome arms exhibited homology with six pairs of LRE macrochromosomes and eight pairs of EQU macrochromosomes. Furthermore, the genes mapped to microchromosomes of three species were all located on chicken microchromosomes or chromosome 4p. No reciprocal translocations were found in the species, and their karyotypic differences were caused by: low frequencies of interchromosomal rearrangements, such as tandem fusions, or centric fissions/fusions between macrochromosomes and between macro- and microchromosomes; and intrachromosomal rearrangements, such as paracentric inversions or centromere repositioning. The chromosomal rearrangements that occurred in macrochromosomes of the Varanus lineage were also identified through comparative cytogenetic mapping of V. salvator macromaculatus and V. exanthematicus. Morphologic differences in chromosomes 6-8 between the two species could have resulted from pericentric inversion or centromere repositioning. © 2013 Springer Science+Business Media Dordrecht.},
note = {cited By 43},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The water monitor lizard (Varanus salvator macromaculatus (VSA), Platynota) has a chromosome number of 2n = 40: its karyotype consists of 16 macrochromosomes and 24 microchromosomes. To delineate the process of karyotype evolution in V. salvator macromaculatus, we constructed a cytogenetic map with 86 functional genes and compared it with those of the butterfly lizard (Leiolepis reevesii rubritaeniata (LRE); 2n = 36) and Japanese four-striped rat snake (Elaphe quadrivirgata (EQU); 2n = 36), members of the Toxicofera clade. The syntenies and gene orders of macrochromosomes were highly conserved between these species except for several chromosomal rearrangements: eight pairs of VSA macrochromosomes and/or chromosome arms exhibited homology with six pairs of LRE macrochromosomes and eight pairs of EQU macrochromosomes. Furthermore, the genes mapped to microchromosomes of three species were all located on chicken microchromosomes or chromosome 4p. No reciprocal translocations were found in the species, and their karyotypic differences were caused by: low frequencies of interchromosomal rearrangements, such as tandem fusions, or centric fissions/fusions between macrochromosomes and between macro- and microchromosomes; and intrachromosomal rearrangements, such as paracentric inversions or centromere repositioning. The chromosomal rearrangements that occurred in macrochromosomes of the Varanus lineage were also identified through comparative cytogenetic mapping of V. salvator macromaculatus and V. exanthematicus. Morphologic differences in chromosomes 6-8 between the two species could have resulted from pericentric inversion or centromere repositioning. © 2013 Springer Science+Business Media Dordrecht.
103.
Chaiprasertsri, N.; Uno, Y.; Peyachoknagul, S.; Prakhongcheep, O.; Baicharoen, S.; Charernsuk, S.; Nishida, C.; Matsuda, Y.; Koga, A.; Srikulnath, K.
Highly species-specific centromeric repetitive DNA sequences in lizards: Molecular cytogenetic characterization of a novel family of satellite DNA sequences isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota) Journal Article
In: Journal of Heredity, vol. 104, no. 6, pp. 798-806, 2013, ISSN: 00221503, (cited By 21).
@article{Chaiprasertsri2013798,
title = {Highly species-specific centromeric repetitive DNA sequences in lizards: Molecular cytogenetic characterization of a novel family of satellite DNA sequences isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota)},
author = {N. Chaiprasertsri and Y. Uno and S. Peyachoknagul and O. Prakhongcheep and S. Baicharoen and S. Charernsuk and C. Nishida and Y. Matsuda and A. Koga and K. Srikulnath},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886312453&doi=10.1093%2fjhered%2fest061&partnerID=40&md5=10413a03652e4340576b679db59bbb18},
doi = {10.1093/jhered/est061},
issn = {00221503},
year = {2013},
date = {2013-01-01},
journal = {Journal of Heredity},
volume = {104},
number = {6},
pages = {798-806},
abstract = {Two novel repetitive DNA sequences, VSAREP1 and VSAREP2, were isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota) and characterized using molecular cytogenetics. The respective lengths and guanine-cytosine (GC) contents of the sequences were 190bp and 57.5% for VSAREP1 and 185bp and 59.7% for VSAREP2, and both elements were tandemly arrayed as satellite DNA in the genome. VSAREP1 and VSAREP2 were each located at the C-positive heterochromatin in the pericentromeric region of chromosome 2q, the centromeric region of chromosome 5, and 3 pairs of microchromosomes. This suggests that genomic compartmentalization between macro- and microchromosomes might not have occurred in the centromeric repetitive sequences of V. salvator macromaculatus. These 2 sequences did only hybridize to genomic DNA of V. salvator macromaculatus, but no signal was observed even for other squamate reptiles, including Varanus exanthematicus, which is a closely related species of V. salvator macromaculatus. These results suggest that these sequences were differentiated rapidly or were specifically amplified in the V. salvator macromaculatus genome. © The American Genetic Association 2013. All rights reserved.},
note = {cited By 21},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Two novel repetitive DNA sequences, VSAREP1 and VSAREP2, were isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota) and characterized using molecular cytogenetics. The respective lengths and guanine-cytosine (GC) contents of the sequences were 190bp and 57.5% for VSAREP1 and 185bp and 59.7% for VSAREP2, and both elements were tandemly arrayed as satellite DNA in the genome. VSAREP1 and VSAREP2 were each located at the C-positive heterochromatin in the pericentromeric region of chromosome 2q, the centromeric region of chromosome 5, and 3 pairs of microchromosomes. This suggests that genomic compartmentalization between macro- and microchromosomes might not have occurred in the centromeric repetitive sequences of V. salvator macromaculatus. These 2 sequences did only hybridize to genomic DNA of V. salvator macromaculatus, but no signal was observed even for other squamate reptiles, including Varanus exanthematicus, which is a closely related species of V. salvator macromaculatus. These results suggest that these sequences were differentiated rapidly or were specifically amplified in the V. salvator macromaculatus genome. © The American Genetic Association 2013. All rights reserved.
104.
Prakhongcheep, O.; Chaiprasertsri, N.; Terada, S.; Hirai, Y.; Srikulnath, K.; Hirai, H.; Koga, A.
Heterochromatin blocks constituting the entire short arms of acrocentric chromosomes of Azara's owl monkey: Formation processes inferred from chromosomal locations Journal Article
In: DNA Research, vol. 20, no. 5, pp. 461-470, 2013, ISSN: 13402838, (cited By 18).
@article{Prakhongcheep2013461,
title = {Heterochromatin blocks constituting the entire short arms of acrocentric chromosomes of Azara's owl monkey: Formation processes inferred from chromosomal locations},
author = {O. Prakhongcheep and N. Chaiprasertsri and S. Terada and Y. Hirai and K. Srikulnath and H. Hirai and A. Koga},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84885119656&doi=10.1093%2fdnares%2fdst023&partnerID=40&md5=16064c3c5435ed9dbfc233802c056ed1},
doi = {10.1093/dnares/dst023},
issn = {13402838},
year = {2013},
date = {2013-01-01},
journal = {DNA Research},
volume = {20},
number = {5},
pages = {461-470},
abstract = {Centromeres and telomeres of higher eukaryotes generally contain repetitive sequences, which often form pericentric or subtelomeric heterochromatin blocks. C-banding analysis of chromosomes of Azara's owl monkey, a primate species, showed that the short arms of acrocentric chromosomes consist mostly or solely of constitutive heterochromatin. The purpose of the present study was to determine which category, pericentric, or subtelomeric is most appropriate for this heterochromatin, and to infer its formation processes. We cloned and sequenced its DNA component, finding it to be a tandem repeat sequence comprising 187-bp repeat units, which we named OwlRep. Subsequent hybridization analyses revealed that OwlRep resides in the pericentric regions of a small number of metacentric chromosomes, in addition to the short arms of acrocentric chromosomes. Further, in the pericentric regions of the acrocentric chromosomes, OwlRep was observed on the short-arm side only. This distribution pattern of OwlRep among chromosomes can be simply and sufficiently explained by assuming (i) OwlRep was transferred from chromosome to chromosome by the interaction of pericentric heterochromatin, and (ii) it was amplified there as subtelomeric heterochromatin. OwlRep carries several direct and inverted repeats within its repeat units. This complex structure may lead to a higher frequency of chromosome scission and may thus be a factor in the unique distribution pattern among chromosomes. Neither OwlRep nor similar sequences were found in the genomes of the other New World monkey species we examined, suggesting that OwlRep underwent rapid amplification after the divergence of the owl monkey lineage from lineages of the other species. © The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.},
note = {cited By 18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Centromeres and telomeres of higher eukaryotes generally contain repetitive sequences, which often form pericentric or subtelomeric heterochromatin blocks. C-banding analysis of chromosomes of Azara's owl monkey, a primate species, showed that the short arms of acrocentric chromosomes consist mostly or solely of constitutive heterochromatin. The purpose of the present study was to determine which category, pericentric, or subtelomeric is most appropriate for this heterochromatin, and to infer its formation processes. We cloned and sequenced its DNA component, finding it to be a tandem repeat sequence comprising 187-bp repeat units, which we named OwlRep. Subsequent hybridization analyses revealed that OwlRep resides in the pericentric regions of a small number of metacentric chromosomes, in addition to the short arms of acrocentric chromosomes. Further, in the pericentric regions of the acrocentric chromosomes, OwlRep was observed on the short-arm side only. This distribution pattern of OwlRep among chromosomes can be simply and sufficiently explained by assuming (i) OwlRep was transferred from chromosome to chromosome by the interaction of pericentric heterochromatin, and (ii) it was amplified there as subtelomeric heterochromatin. OwlRep carries several direct and inverted repeats within its repeat units. This complex structure may lead to a higher frequency of chromosome scission and may thus be a factor in the unique distribution pattern among chromosomes. Neither OwlRep nor similar sequences were found in the genomes of the other New World monkey species we examined, suggesting that OwlRep underwent rapid amplification after the divergence of the owl monkey lineage from lineages of the other species. © The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
105.
Prakhongcheep, O.; Hirai, Y.; Hara, T.; Srikulnath, K.; Hirai, H.; Koga, A.
Two types of alpha satellite DNA in distinct chromosomal locations in Azara's owl monkey Journal Article
In: DNA Research, vol. 20, no. 3, pp. 235-240, 2013, ISSN: 13402838, (cited By 23).
@article{Prakhongcheep2013235,
title = {Two types of alpha satellite DNA in distinct chromosomal locations in Azara's owl monkey},
author = {O. Prakhongcheep and Y. Hirai and T. Hara and K. Srikulnath and H. Hirai and A. Koga},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884181744&doi=10.1093%2fdnares%2fdst004&partnerID=40&md5=dca6079ee15735c0d323ef9f9f28311c},
doi = {10.1093/dnares/dst004},
issn = {13402838},
year = {2013},
date = {2013-01-01},
journal = {DNA Research},
volume = {20},
number = {3},
pages = {235-240},
abstract = {Alpha satellite DNA is a repetitive sequence known to be a major DNA component of centromeres in primates (order Primates). New World monkeys form one major taxon (parvorder Platyrrhini) of primates, and their alpha satellite DNA is known to comprise repeat units of around 340 bp. In one species (Azara's owl monkey Aotus azarae) of this taxon, we identified two types of alpha satellite DNA consisting of 185- and 344-bp repeat units that we designated as OwlAlp1 and OwlAlp2, respectively. OwlAlp2 exhibits similarity throughout its entire sequence to the alpha satellite DNA of other New World monkeys. The chromosomal locations of the two types of sequence are markedly distinct: OwlAlp1 was observed at the centromeric constrictions, whereas OwlAlp2 was found in the pericentric regions. From these results, we inferred that OwlAlp1 was derived from OwlAlp2 and rapidly replaced OwlAlp2 as the principal alpha satellite DNA on a short time scale at the speciation level. A less likely alternative explanation is also discussed. © The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.},
note = {cited By 23},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Alpha satellite DNA is a repetitive sequence known to be a major DNA component of centromeres in primates (order Primates). New World monkeys form one major taxon (parvorder Platyrrhini) of primates, and their alpha satellite DNA is known to comprise repeat units of around 340 bp. In one species (Azara's owl monkey Aotus azarae) of this taxon, we identified two types of alpha satellite DNA consisting of 185- and 344-bp repeat units that we designated as OwlAlp1 and OwlAlp2, respectively. OwlAlp2 exhibits similarity throughout its entire sequence to the alpha satellite DNA of other New World monkeys. The chromosomal locations of the two types of sequence are markedly distinct: OwlAlp1 was observed at the centromeric constrictions, whereas OwlAlp2 was found in the pericentric regions. From these results, we inferred that OwlAlp1 was derived from OwlAlp2 and rapidly replaced OwlAlp2 as the principal alpha satellite DNA on a short time scale at the speciation level. A less likely alternative explanation is also discussed. © The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
106.
Islam, F. B.; Ishishita, S.; Uno, Y.; Mollah, M. B. R.; Srikulnath, K.; Matsuda, Y.
Male hybrid sterility in the mule duck is associated with meiotic arrest in primary spermatocytes Journal Article
In: Journal of Poultry Science, vol. 50, no. 4, pp. 311-320, 2013, ISSN: 13467395, (cited By 11).
@article{Islam2013311,
title = {Male hybrid sterility in the mule duck is associated with meiotic arrest in primary spermatocytes},
author = {F. B. Islam and S. Ishishita and Y. Uno and M. B. R. Mollah and K. Srikulnath and Y. Matsuda},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886384271&doi=10.2141%2fjpsa.0130011&partnerID=40&md5=27c6733c2e0d909d5b8c345fea06fc4c},
doi = {10.2141/jpsa.0130011},
issn = {13467395},
year = {2013},
date = {2013-01-01},
journal = {Journal of Poultry Science},
volume = {50},
number = {4},
pages = {311-320},
abstract = {Hybrid sterility is a postzygotic reproductive isolation mechanism that prevents successful interbreeding between different species. The mule duck, an intergeneric F1 hybrid between the domestic duck (Anas platyrhynchos) and Muscovy duck (Cairina moschata), displays sterility with gametogenesis failure in both sexes. Although the F1 hybrid male is known to exhibit large-sized testes that produce no sperm, the spermatogenic phenotype has not been well described. In this study, we revealed the abnormal meiotic phenotype of the F1 hybrid spermatocytes and dissimilarity in the karyotypes between the two parental species. Histological examination of the F1 hybrid testis showed the accumulation of primary spermatocytes with irregular highly condensed chromosomes in the seminiferous epithelium, whereas secondary spermatocytes and postmeiotic cells were absent and many testicular cells undergoing apoptosis were present. Cytogenetic analyses of spermatogenic cells from the F1 hybrid male revealed that meiosis succeeded in entering pachytene, but failed to progress beyond diakinesis-metaphase I in primary spermatocytes, and that a number of degenerated spermatocytes were present at pachytene. Karyological observations showed morphological differences in chromosome 1 and the Z chromosome between the parental species. These results collectively suggest that the main cause of abnormal spermatogenesis in the F1 hybrid is pachytene and/or metaphase I arrest, which possibly resulted from the failure of homologous chromosome pairing, recombination, and subsequent chromosome segregation due to chromosomal incompatibility between the parental species. © 2013, Japan Poultry Science Association.},
note = {cited By 11},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hybrid sterility is a postzygotic reproductive isolation mechanism that prevents successful interbreeding between different species. The mule duck, an intergeneric F1 hybrid between the domestic duck (Anas platyrhynchos) and Muscovy duck (Cairina moschata), displays sterility with gametogenesis failure in both sexes. Although the F1 hybrid male is known to exhibit large-sized testes that produce no sperm, the spermatogenic phenotype has not been well described. In this study, we revealed the abnormal meiotic phenotype of the F1 hybrid spermatocytes and dissimilarity in the karyotypes between the two parental species. Histological examination of the F1 hybrid testis showed the accumulation of primary spermatocytes with irregular highly condensed chromosomes in the seminiferous epithelium, whereas secondary spermatocytes and postmeiotic cells were absent and many testicular cells undergoing apoptosis were present. Cytogenetic analyses of spermatogenic cells from the F1 hybrid male revealed that meiosis succeeded in entering pachytene, but failed to progress beyond diakinesis-metaphase I in primary spermatocytes, and that a number of degenerated spermatocytes were present at pachytene. Karyological observations showed morphological differences in chromosome 1 and the Z chromosome between the parental species. These results collectively suggest that the main cause of abnormal spermatogenesis in the F1 hybrid is pachytene and/or metaphase I arrest, which possibly resulted from the failure of homologous chromosome pairing, recombination, and subsequent chromosome segregation due to chromosomal incompatibility between the parental species. © 2013, Japan Poultry Science Association.
2012
107.
Srikulnath, K.; Thongpan, A.; Suputtitada, S.; Apisitwanich, S.
New haplotype of the complete mitochondrial genome of Crocodylus siamensis and its species-specific DNA markers: Distinguishing C. siamensis from C. porosus in Thailand Journal Article
In: Molecular Biology Reports, vol. 39, no. 4, pp. 4709-4717, 2012, ISSN: 03014851, (cited By 19).
@article{Srikulnath20124709,
title = {New haplotype of the complete mitochondrial genome of Crocodylus siamensis and its species-specific DNA markers: Distinguishing C. siamensis from C. porosus in Thailand},
author = {K. Srikulnath and A. Thongpan and S. Suputtitada and S. Apisitwanich},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863092523&doi=10.1007%2fs11033-011-1263-7&partnerID=40&md5=be1d4218439e674b1d611909b4ea3273},
doi = {10.1007/s11033-011-1263-7},
issn = {03014851},
year = {2012},
date = {2012-01-01},
journal = {Molecular Biology Reports},
volume = {39},
number = {4},
pages = {4709-4717},
abstract = {Based on molecular phylogeny of available complete mitochondrial DNA (mtDNA) genome sequences reveals that Crocodylus siamensis and C. porosus are closely related species. Yet, the sequence divergence of their mtDNA showed only a few values under conspecific level. In this study, a new haplotype (haplotype2, EF581859) of the complete mtDNA genome of Siamese crocodile (C. siamensis) was determined. The genome organization, which appeared to be highly similar to haplotype1 (DQ353946) mtDNA genome of C. siamensis, was 16,814 bp in length. However, the sequence divergence between the two genomes differed by around 7-10 and 0.7-2.1% for the haplotype1 between C. siamensis and C. porosus (AJ810453). These results were consistent with the phylogenetic relationship among the three genomes, suggesting that C. siamensis haplotype1 mtDNA genome might be the hybrid or the intraspecific variation of C. porosus. On the other hand, our specimen was found to be a true C. siamensis. Simultaneously, the seven speciesspecific DNA markers designed based on the distinctive site between haplotype2 mtDNA sequences of C. siamensis and haplotype1 mtDNA sequence of C. siamensis- C. porosus were successfully used to distinguish C. siamensis from C. porosus. These effective markers could be used primarily for rapid and accurate species identification in population, ecology and conservation studies. © Springer Science+Business Media B.V. 2011.},
note = {cited By 19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Based on molecular phylogeny of available complete mitochondrial DNA (mtDNA) genome sequences reveals that Crocodylus siamensis and C. porosus are closely related species. Yet, the sequence divergence of their mtDNA showed only a few values under conspecific level. In this study, a new haplotype (haplotype2, EF581859) of the complete mtDNA genome of Siamese crocodile (C. siamensis) was determined. The genome organization, which appeared to be highly similar to haplotype1 (DQ353946) mtDNA genome of C. siamensis, was 16,814 bp in length. However, the sequence divergence between the two genomes differed by around 7-10 and 0.7-2.1% for the haplotype1 between C. siamensis and C. porosus (AJ810453). These results were consistent with the phylogenetic relationship among the three genomes, suggesting that C. siamensis haplotype1 mtDNA genome might be the hybrid or the intraspecific variation of C. porosus. On the other hand, our specimen was found to be a true C. siamensis. Simultaneously, the seven speciesspecific DNA markers designed based on the distinctive site between haplotype2 mtDNA sequences of C. siamensis and haplotype1 mtDNA sequence of C. siamensis- C. porosus were successfully used to distinguish C. siamensis from C. porosus. These effective markers could be used primarily for rapid and accurate species identification in population, ecology and conservation studies. © Springer Science+Business Media B.V. 2011.
2011
108.
Unajak, S.; Meesawat, P.; Anyamaneeratch, K.; Anuwareepong, D.; Srikulnath, K.; Choowongkomon, K.
Identification of species (meat and blood samples)using nested-PCR analysis of mitochondrial DNA Journal Article
In: African Journal of Biotechnology, vol. 10, no. 29, pp. 5670-5676, 2011, ISSN: 16845315, (cited By 29).
@article{Unajak20115670,
title = {Identification of species (meat and blood samples)using nested-PCR analysis of mitochondrial DNA},
author = {S. Unajak and P. Meesawat and K. Anyamaneeratch and D. Anuwareepong and K. Srikulnath and K. Choowongkomon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79959759560&partnerID=40&md5=88b21decd92721d941bb222bf42b5746},
issn = {16845315},
year = {2011},
date = {2011-01-01},
journal = {African Journal of Biotechnology},
volume = {10},
number = {29},
pages = {5670-5676},
abstract = {Crocodile meat product is an alternative protein source. Although, crocodile meat is more expensive, itstaste is similar to that of chicken and fish. The authentication of commercial meat species is importantfor consumer's confidence. In this study, sensitive and specific method multiplex nested-PCR wasapplied to identify commercial meat species. Dried blood was used as an alternative DNA source fordetection. The detection sensitivity was enhanced by primers specifically designed to encompass themitochondrial Cytochrome b and NADH dehydrogenase 5/6 genes. The specificity and sensitivity ofmultiplex PCR system were tested. Different lengths of specific nested-PCR products were detected tobe 350, 570, 750 and 1000 bp for chicken, pig, cow, and crocodile, respectively. The system alloweddetection with as little as 5 nanogram of DNA from either meat or blood sample. Detection sensitivity ofindividual species was improved, enabling the detection of DNA with as little as 1 picogram. Crossreaction was not detected among the tested species. It was shown that the multiplex-PCR assayenhanced the sensitivity of routine species identification and allowed the use of blood as an alternativeDNA source for detection. © 2011 Academic Journals.},
note = {cited By 29},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Crocodile meat product is an alternative protein source. Although, crocodile meat is more expensive, itstaste is similar to that of chicken and fish. The authentication of commercial meat species is importantfor consumer's confidence. In this study, sensitive and specific method multiplex nested-PCR wasapplied to identify commercial meat species. Dried blood was used as an alternative DNA source fordetection. The detection sensitivity was enhanced by primers specifically designed to encompass themitochondrial Cytochrome b and NADH dehydrogenase 5/6 genes. The specificity and sensitivity ofmultiplex PCR system were tested. Different lengths of specific nested-PCR products were detected tobe 350, 570, 750 and 1000 bp for chicken, pig, cow, and crocodile, respectively. The system alloweddetection with as little as 5 nanogram of DNA from either meat or blood sample. Detection sensitivity ofindividual species was improved, enabling the detection of DNA with as little as 1 picogram. Crossreaction was not detected among the tested species. It was shown that the multiplex-PCR assayenhanced the sensitivity of routine species identification and allowed the use of blood as an alternativeDNA source for detection. © 2011 Academic Journals.
109.
Srikulnath, K.; Uno, Y.; Matsubara, K.; Thongpan, A.; Suputtitada, S.; Apisitwanich, S.; Nishida, C.; Matsuda, Y.
Chromosomal localization of the 18S-28S and 5s rRNA genes and (TTAGGG)nsequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata) Journal Article
In: Genetics and Molecular Biology, vol. 34, no. 4, pp. 582-586, 2011, ISSN: 14154757, (cited By 20).
@article{Srikulnath2011582,
title = {Chromosomal localization of the 18S-28S and 5s rRNA genes and (TTAGGG)nsequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata)},
author = {K. Srikulnath and Y. Uno and K. Matsubara and A. Thongpan and S. Suputtitada and S. Apisitwanich and C. Nishida and Y. Matsuda},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-82355182925&doi=10.1590%2fs1415-47572011005000042&partnerID=40&md5=0fbb8b83bb1c9753e1c0208edb548865},
doi = {10.1590/s1415-47572011005000042},
issn = {14154757},
year = {2011},
date = {2011-01-01},
journal = {Genetics and Molecular Biology},
volume = {34},
number = {4},
pages = {582-586},
publisher = {Brazilian Journal of Genetics},
abstract = {Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)nsequences. The karyotype of L. b. bellianawas 2n = 36, whereas that ofL. boehmeiwas 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)nsequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that inL. boehmeitelomere-to-telomere fusion probably occurred between chromosome 1 and a micro chromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telemetric sequence signals in chromosome 1 ofL. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)nsequence or that there may have been a gradual loss of the repeat sequences during chromosomal evolution. © 2011, Sociedade Brasileira de Genética.},
note = {cited By 20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)nsequences. The karyotype of L. b. bellianawas 2n = 36, whereas that ofL. boehmeiwas 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)nsequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that inL. boehmeitelomere-to-telomere fusion probably occurred between chromosome 1 and a micro chromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telemetric sequence signals in chromosome 1 ofL. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)nsequence or that there may have been a gradual loss of the repeat sequences during chromosomal evolution. © 2011, Sociedade Brasileira de Genética.
2010
110.
Srikulnath, K.; Matsubara, K.; Uno, Y.; Thongpan, A.; Suputtitada, S.; Nishida, C.; Matsuda, Y.; Apisitwanich, S.
Genetic relationship of three butterfly lizard species (Leiolepis reevesii rubritaeniata, Leiolepis belliana belliana, Leiolepis boehmei, Agamidae, Squamata) inferred from nuclear gene sequence analyses Journal Article
In: Kasetsart Journal - Natural Science, vol. 44, no. 3, pp. 424-435, 2010, ISSN: 00755192, (cited By 13).
@article{Srikulnath2010424,
title = {Genetic relationship of three butterfly lizard species (Leiolepis reevesii rubritaeniata, Leiolepis belliana belliana, Leiolepis boehmei, Agamidae, Squamata) inferred from nuclear gene sequence analyses},
author = {K. Srikulnath and K. Matsubara and Y. Uno and A. Thongpan and S. Suputtitada and C. Nishida and Y. Matsuda and S. Apisitwanich},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954451175&partnerID=40&md5=9ed077e27d7b5f7cd76a06c27d474b79},
issn = {00755192},
year = {2010},
date = {2010-01-01},
journal = {Kasetsart Journal - Natural Science},
volume = {44},
number = {3},
pages = {424-435},
abstract = {The genetic relationship was investigated of three butterfly lizard species (Leiolepis reevesii rubritaeniata, L. belliana belliana and L. boehmei) selectively inhabiting Thailand. The findings were based on RAG1 and C-mos gene analyses. The DNA sequences were also compared with the other squamate reptiles. The analysis strongly supported that L. reevesii rubritaeniata was related more closely to L. belliana belliana than to L. boehmei. The phylogenetic position of Leiolepis spp., however, was contentious with regard to its relationship among the Leiolepidinae, Agaminae and Chamaeleonidae, which suggested that their phylogeny remains uncertain.},
note = {cited By 13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The genetic relationship was investigated of three butterfly lizard species (Leiolepis reevesii rubritaeniata, L. belliana belliana and L. boehmei) selectively inhabiting Thailand. The findings were based on RAG1 and C-mos gene analyses. The DNA sequences were also compared with the other squamate reptiles. The analysis strongly supported that L. reevesii rubritaeniata was related more closely to L. belliana belliana than to L. boehmei. The phylogenetic position of Leiolepis spp., however, was contentious with regard to its relationship among the Leiolepidinae, Agaminae and Chamaeleonidae, which suggested that their phylogeny remains uncertain.
2009
111.
Srikulnath, K.; Nishida, C.; Matsubara, K.; Uno, Y.; Thongpan, A.; Suputtitada, S.; Apisitwanich, S.; Matsuda, Y.
Karyotypic evolution in squamate reptiles: Comparative gene mapping revealed highly conserved linkage homology between the butterfly lizard (Leiolepis reevesii rubritaeniata, Agamidae, Lacertilia) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae, Serpentes) Journal Article
In: Chromosome Research, vol. 17, no. 8, pp. 975-986, 2009, ISSN: 09673849, (cited By 57).
@article{Srikulnath2009975,
title = {Karyotypic evolution in squamate reptiles: Comparative gene mapping revealed highly conserved linkage homology between the butterfly lizard (Leiolepis reevesii rubritaeniata, Agamidae, Lacertilia) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae, Serpentes)},
author = {K. Srikulnath and C. Nishida and K. Matsubara and Y. Uno and A. Thongpan and S. Suputtitada and S. Apisitwanich and Y. Matsuda},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77950907912&doi=10.1007%2fs10577-009-9101-7&partnerID=40&md5=127f7750a05b30efb2374f4f34f2c3ee},
doi = {10.1007/s10577-009-9101-7},
issn = {09673849},
year = {2009},
date = {2009-01-01},
journal = {Chromosome Research},
volume = {17},
number = {8},
pages = {975-986},
abstract = {The butterfly lizard (Leiolepis reevesii rubritaeniata) has the diploid chromosome number of 2n=36, comprising two distinctive components, macrochromosomes and microchromosomes. To clarify the conserved linkage homology between lizard and snake chromosomes and to delineate the process of karyotypic evolution in Squamata, we constructed a cytogenetic map of L. reevesii rubritaeniata with 54 functional genes and compared it with that of the Japanese four-striped rat snake (E. quadrivirgata},
note = {cited By 57},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The butterfly lizard (Leiolepis reevesii rubritaeniata) has the diploid chromosome number of 2n=36, comprising two distinctive components, macrochromosomes and microchromosomes. To clarify the conserved linkage homology between lizard and snake chromosomes and to delineate the process of karyotypic evolution in Squamata, we constructed a cytogenetic map of L. reevesii rubritaeniata with 54 functional genes and compared it with that of the Japanese four-striped rat snake (E. quadrivirgata
112.
Srikulnath, K.; Matsubara, K.; Uno, Y.; Thongpan, A.; Suputtitada, S.; Apisitwanich, S.; Matsuda, Y.; Nishida, C.
Karyological characterization of the butterfly lizard (leiolepis reevesii rubritaeniata, agamidae, squamata) by molecular cytogenetic approach Journal Article
In: Cytogenetic and Genome Research, vol. 125, no. 3, pp. 213-223, 2009, ISSN: 14248581, (cited By 53).
@article{Srikulnath2009213,
title = {Karyological characterization of the butterfly lizard (leiolepis reevesii rubritaeniata, agamidae, squamata) by molecular cytogenetic approach},
author = {K. Srikulnath and K. Matsubara and Y. Uno and A. Thongpan and S. Suputtitada and S. Apisitwanich and Y. Matsuda and C. Nishida},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349141834&doi=10.1159%2f000230005&partnerID=40&md5=d83506ece928914ede96d495f4ca2cee},
doi = {10.1159/000230005},
issn = {14248581},
year = {2009},
date = {2009-01-01},
journal = {Cytogenetic and Genome Research},
volume = {125},
number = {3},
pages = {213-223},
abstract = {Karyological characterization of the butterfly lizard (Leiolepis reevesii rubritaeniata) was performed by conventional Giemsa staining, Ag-NOR banding, FISH with the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences, and CGH. The karyotype was composed of 2 distinct components, macrochromosomes and microchromosomes, and the chromosomal constitution was 2n = 2x = 36 (L 4m + L2sm + M2 m + S4m + 24 microchromosomes). NORs and the 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, and the 5S rRNA genes were localized to the pericentromeric region of chromosome 6. Hybridization signals of (TTAGGG)n sequences were observed at the telomeric ends of all chromosomes and interstitially at the same position as the 18S-28S rRNA genes, suggesting that in the Leiolepinae tandem fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located. CGH analysis, however, failed to identify sex chromosomes, suggesting that this species may have a TSD system or exhibit GSD with morphologically undetectable cryptic sex chromosomes. Homologues of 6 chicken Z-linked genes (ACO1/IREBP, ATP5A1, CHD1, DMRT1, GHR, RPS6) were all mapped to chromosome 2p in the same order as on the snake chromosome 2p. © 2009 S. Karger AG, Basel.},
note = {cited By 53},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Karyological characterization of the butterfly lizard (Leiolepis reevesii rubritaeniata) was performed by conventional Giemsa staining, Ag-NOR banding, FISH with the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences, and CGH. The karyotype was composed of 2 distinct components, macrochromosomes and microchromosomes, and the chromosomal constitution was 2n = 2x = 36 (L 4m + L2sm + M2 m + S4m + 24 microchromosomes). NORs and the 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, and the 5S rRNA genes were localized to the pericentromeric region of chromosome 6. Hybridization signals of (TTAGGG)n sequences were observed at the telomeric ends of all chromosomes and interstitially at the same position as the 18S-28S rRNA genes, suggesting that in the Leiolepinae tandem fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located. CGH analysis, however, failed to identify sex chromosomes, suggesting that this species may have a TSD system or exhibit GSD with morphologically undetectable cryptic sex chromosomes. Homologues of 6 chicken Z-linked genes (ACO1/IREBP, ATP5A1, CHD1, DMRT1, GHR, RPS6) were all mapped to chromosome 2p in the same order as on the snake chromosome 2p. © 2009 S. Karger AG, Basel.