@article{Laopichienpong20174661,

title = {A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes},

author = {N. Laopichienpong and P. Tawichasri and L. Chanhome and R. Phatcharakullawarawat and W. Singchat and A. Kantachumpoo and N. Muangmai and S. Suntrarachun and K. Matsubara and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019604014&doi=10.1002%2fece3.3057&partnerID=40&md5=00767828f9601e759a9dc9a9ee0708e0},

doi = {10.1002/ece3.3057},

issn = {20457758},

year  = {2017},

date = {2017-01-01},

journal = {Ecology and Evolution},

volume = {7},

number = {13},

pages = {4661-4669},

publisher = {John Wiley and Sons Ltd},

abstract = {Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in difficulties in the management of their breeding programs. In this study, the nucleotide sequences of gametologous genes (CTNNB1 and WAC genes) were used for the development of molecular sexing markers in caenophidian snakes. Two candidate markers were developed with the two primer sets, and successfully amplified by a single band on the agarose gel in male (ZZ) and two bands, differing in fragment sizes, in female (ZW) of 16 caenophidian snakes for CTNNB1 and 12 caenophidian snakes for WAC. Another candidate marker was developed with the primer set to amplify the specific sequence for CTNNB1W homolog, and the PCR products were successfully obtained in a female-specific 250-bp DNA bands. The three candidate PCR sexing markers provide a simple sex identification method based on the amplification of gametologous genes, and they can be used to facilitate effective caenophidian snake conservation and management programs. © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.},

note = {cited By 12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ThongtamnaAyudhaya2017198,

title = {Unveiling cryptic diversity of the anemonefish genera Amphiprion and Premnas (Perciformes: Pomacentridae) in Thailand with mitochondrial DNA barcodes},

author = {P. Thongtam na Ayudhaya and N. Muangmai and N. Banjongsat and W. Singchat and S. Janekitkarn and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028653591&doi=10.1016%2fj.anres.2017.07.001&partnerID=40&md5=6f73dac252fbced04754a37b13569f0e},

doi = {10.1016/j.anres.2017.07.001},

issn = {24681458},

year  = {2017},

date = {2017-01-01},

journal = {Agriculture and Natural Resources},

volume = {51},

number = {3},

pages = {198-205},

publisher = {Elsevier B.V.},

abstract = {The genera Amphiprion and Premnas comprise the common anemonefish that are widely distributed in tropical areas. Species identification of these two genera is difficult due to high, intraspecific, morphological variation. Recently, DNA barcoding has been employed as an efficient tool that uses a short genetic marker in an organism's DNA to enable the identification and recognition of cryptic species. This study applied three regions of mitochondrial DNA—cytochrome c oxidase I (COI), cytochrome b (Cytb) and 16S rRNA—as DNA barcodes for species identification of seven species of Amphiprion and one species of Premnas in Thailand. Three species-delimitation methods—general mixed Yule-coalescent (GMYC), automatic barcoding gap detection (ABGD) and a Bayesian implementation of the Poisson tree processes model (bPTP)—were also used to estimate the number of species. An overlap was found between the intra- and inter-specific genetic divergence values in Cytb and 16S rRNA, but not for the COI data. This indicated that COI was the most effective for identifying different anemonefish species. A three-gene phylogenetic analysis and species-delimitation methods based on both COI and Cytb data suggested cryptic diversity in Amphiprion clarkii, A. percula, A. ocellaris and Premnas biaculeatus. Different distributions were found also for two cryptic species of A. clarkia—one restricted to the Gulf of Thailand and the other to the Andaman Sea. The results confirmed the efficiency of COI as a suitable marker for species identification of anemonefish. © 2017 Kasetsart University},

note = {cited By 12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Laopichienpong2017142,

title = {Evolutionary dynamics of the gametologous CTNNB1 gene on the Z and W chromosomes of snakes},

author = {N. Laopichienpong and N. Muangmai and L. Chanhome and S. Suntrarachun and P. Twilprawat and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85015900644&doi=10.1093%2fjhered%2fesw074&partnerID=40&md5=2d70bbba99c96bd3a71905a8a9d11abb},

doi = {10.1093/jhered/esw074},

issn = {00221503},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Heredity},

volume = {108},

number = {2},

pages = {142-151},

publisher = {Oxford University Press},

abstract = {Snakes exhibit genotypic sex determination with female heterogamety (ZZ males and ZW females), and the state of sex chromosome differentiation also varies among lineages. To investigate the evolutionary history of homologous genes located in the nonrecombining region of differentiated sex chromosomes in snakes, partial sequences of the gametologous CTNNB1 gene were analyzed for 12 species belonging to henophid (Cylindrophiidae, Xenopeltidae, and Pythonidae) and caenophid snakes (Viperidae, Elapidae, and Colubridae). Nonsynonymous/synonymous substitution ratios (Ka/Ks) in coding sequences were low (Ka/Ks < 1) between CTNNB1Z and CTNNB1W, suggesting that these 2 genes may have similar functional properties. However, frequencies of intron sequence substitutions and insertion-deletions were higher in CTNNB1Z than CTNNB1W, suggesting that Z-linked sequences evolved faster than W-linked sequences. Molecular phylogeny based on both intron and exon sequences showed the presence of 2 major clades: 1) Z-linked sequences of Caenophidia and 2) W-linked sequences of Caenophidia clustered with Z-linked sequences of Henophidia, which suggests that the sequence divergence between CTNNB1Z and CTNNB1W in Caenophidia may have occurred by the cessation of recombination after the split from Henophidia. © The American Genetic Association 2016. All rights reserved.},

note = {cited By 16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Suntronpong2017435,

title = {Karyological characterization and identification of four repetitive element groups (the 18S - 28S rRNA gene, telomeric sequences, microsatellite repeat motifs, Rex retroelements) of the Asian swamp eel (Monopterus albus)},

author = {A. Suntronpong and W. Thapana and P. Twilprawat and O. Prakhongcheep and S. Somyong and N. Muangmai and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028688204&doi=10.3897%2fcompcytogen.v11i3.11739&partnerID=40&md5=09370a1e42887c155daadbf3f12e9ae4},

doi = {10.3897/compcytogen.v11i3.11739},

issn = {19930771},

year  = {2017},

date = {2017-01-01},

journal = {Comparative Cytogenetics},

volume = {11},

number = {3},

pages = {435-462},

publisher = {Pensoft Publishers},

abstract = {Among teleost fishes, Asian swamp eel (Monopterus albus Zuiew, 1793) possesses the lowest chromosome number},

note = {cited By 10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ezaz201794,

title = {Origin of amniote sex chromosomes: An ancestral super-sex chromosome, or common requirements?},

author = {T. Ezaz and K. Srikulnath and J. A. M. Graves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85012144084&doi=10.1093%2fjhered%2fesw053&partnerID=40&md5=8725d4b0cf7819819262735275ae61f5},

doi = {10.1093/jhered/esw053},

issn = {00221503},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Heredity},

volume = {108},

number = {1},

pages = {94-105},

publisher = {Oxford University Press},

abstract = {The diversity of sex chromosomes among amniotes is the product of independent evolution of different systems in different lineages, defined by novel sex-determining genes. Convergent evolution is very common, suggesting that some genes are particularly adept at taking on a sexdetermining role. Comparative gene mapping, and more recently whole genome sequencing, have now turned up other surprising relationships; different regions of the amniote genome that have become sex determining in some taxa seem to share synteny, or share sequence, in others. Is this, after all, evidence that these regions were once linked in a super-sex chromosome that underwent multiple fission in different ways in different amniote lineages? Or does it signify that special properties of sex chromosomes (paucity of active genes, low recombination, epigenetic regulation to achieve dosage compensation) predispose particular chromosomes to a sex-determining role? © The American Genetic Association 2016. All rights reserved.},

note = {cited By 51},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Laopichienpong2016238,

title = {Assessment of snake DNA barcodes based on mitochondrial COI and Cytb genes revealed multiple putative cryptic species in Thailand},

author = {N. Laopichienpong and N. Muangmai and A. Supikamolseni and P. Twilprawat and L. Chanhome and S. Suntrarachun and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992677737&doi=10.1016%2fj.gene.2016.09.017&partnerID=40&md5=79a9f0319ed3192c857726336a3c80de},

doi = {10.1016/j.gene.2016.09.017},

issn = {03781119},

year  = {2016},

date = {2016-01-01},

journal = {Gene},

volume = {594},

number = {2},

pages = {238-247},

publisher = {Elsevier B.V.},

abstract = {DNA barcodes of mitochondrial cytochrome c oxidase I (COI), cytochrome b (Cytb) genes, and their combined data sets were constructed from 35 snake species in Thailand. No barcoding gap was detected in either of the two genes from the observed intra- and interspecific sequence divergences. Intra- and interspecific sequence divergences of the COI gene differed 14 times, with barcode cut-off scores ranging over 2%–4% for threshold values differentiated among most of the different species; the Cytb gene differed 6 times with cut-off scores ranging over 2%–6%. Thirty-five specific nucleotide mutations were also found at interspecific level in the COI gene, identifying 18 snake species, but no specific nucleotide mutation was observed for Cytb in any single species. This suggests that COI barcoding was a better marker than Cytb. Phylogenetic clustering analysis indicated that most species were represented by monophyletic clusters, suggesting that these snake species could be clearly differentiated using COI barcodes. However, the two-marker combination of both COI and Cytb was more effective, differentiating snake species by over 2%–4%, and reducing species numbers in the overlap value between intra- and interspecific divergences. Three species delimitation algorithms (general mixed Yule-coalescent, automatic barcoding gap detection, and statistical parsimony network analysis) were extensively applied to a wide range of snakes based on both barcodes. This revealed cryptic diversity for eleven snake species in Thailand. In addition, eleven accessions from the database previously grouped under the same species were represented at different species level, suggesting either high genetic diversity, or the misidentification of these sequences in the database as a consequence of cryptic species. © 2016 Elsevier B.V.},

note = {cited By 36},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Baicharoen2016267,

title = {Hypervariability of Nucleolus Organizer Regions in Bengal Slow Lorises, Nycticebus bengalensis (Primates, Lorisidae)},

author = {S. Baicharoen and Y. Hirai and K. Srikulnath and U. Kongprom and H. Hirai},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988603431&doi=10.1159%2f000449145&partnerID=40&md5=6f0d80e1ac49018823fdd77a74d7beb5},

doi = {10.1159/000449145},

issn = {14248581},

year  = {2016},

date = {2016-01-01},

journal = {Cytogenetic and Genome Research},

volume = {149},

number = {4},

pages = {267-273},

publisher = {S. Karger AG},

abstract = {Slow lorises are a cryptic species complex, and thus genetic markers are needed to identify distinct evolutionary lineages or species. We examined the nucleolus organizer regions (NORs) of Bengal slow lorises (Nycticebus bengalensis) using FISH with 18S rDNA (rDNA-FISH) and silver nitrate staining (Ag-NOR stain). Ten individuals of the putatively single species N. bengalensis showed higher variability in localization than 3 other congeners, though their overall karyotypes were similar. The rDNA-FISH analysis detected a total of 18 loci, in contrast to previous studies of other slow loris species that revealed far fewer (6-10) loci. Eight of the 18 loci detected in the present analysis were found to be semi-stable localizations at 4 different chromosomes, while 10 were found to be unstable localizations at 5 other chromosomes. The semi-stable locations showed occasional presence/absence of variations for rDNA-FISH, and unstable locations were polymorphic among individuals, contributing to the higher variability of NORs in this taxon. We hypothesize that the larger numbers of rDNA loci found in N. bengalensis were introduced by genomic dispersion through ectopic recombination in association with terminal regions including rDNA. Such differences are potentially very powerful chromosomal markers to be used in species identification and conservation. © 2016 S. Karger AG, Basel.},

note = {cited By 5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vongvanrungruang2016,

title = {Single base substitution causing the fragrant phenotype and development of a type-specific marker in aromatic coconut (Cocos nucifera)},

author = {A. Vongvanrungruang and C. Mongkolsiriwatana and T. Boonkaew and O. Sawatdichaikul and K. Srikulnath and S. Peyachoknagul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989916560&doi=10.4238%2fgmr.15038748&partnerID=40&md5=a5b679568d6506504f6aca4889eaf254},

doi = {10.4238/gmr.15038748},

issn = {16765680},

year  = {2016},

date = {2016-01-01},

journal = {Genetics and Molecular Research},

volume = {15},

number = {3},

publisher = {Fundacao de Pesquisas Cientificas de Ribeirao Preto},

abstract = {The fragrance gene, betaine aldehyde dehydrogenase 2 (Badh2), has been well studied in many plant species. The objectives of this study were to clone Badh2 and compare the sequences between aromatic and non-aromatic coconuts. The complete coding region was cloned from cDNA of both aromatic and non-aromatic coconuts. The nucleotide sequences were highly homologous to Badh2 genes of other plants. Badh2 consisted of a 1512-bp open reading frame encoding 503 amino acids. A single nucleotide difference between aromatic and non-aromatic coconuts resulted in the conversion of alanine (non-aromatic) to proline (aromatic) at position 442, which was the substrate binding site of BADH2. The ring side chain of proline could destabilize the structure leading to a non-functional enzyme. Badh2 genomic DNA was cloned from exon 1 to 4, and from exon 5 to 15 from the two coconut types, except for intron 4 that was very long. The intron sequences of the two coconut groups were highly homologous. No differences in Badh2 expression were found among the tissues of aromatic coconut or between aromatic and non-aromatic coconuts. The amino acid sequences of BADH2 from coconut and other plants were compared and the genetic relationship was analyzed using MEGA 7.0. The phylogenetic tree reconstructed by the Bayesian information criterion consisted of two distinct groups of monocots and dicots. Among the monocots, coconut (Cocos nucifera) and oil palm (Elaeis guineensis) were the most closely related species. A marker for coconut differentiation was developed from one-base substitution site and could be successfully used. © 2016 The Authors.},

note = {cited By 11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Singchat2016,

title = {Genomic alteration in Head and Neck Squamous Cell Carcinoma (HNSCC) cell lines inferred from karyotyping, molecular cytogenetics, and array comparative genomic hybridization},

author = {W. Singchat and E. Hitakomate and B. Rerkarmnuaychoke and A. Suntronpong and B. Fu and W. Bodhisuwan and S. Peyachoknagul and F. Yang and S. Koontongkaew and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983086804&doi=10.1371%2fjournal.pone.0160901&partnerID=40&md5=11fcbd7a253c74b3093987ca53534965},

doi = {10.1371/journal.pone.0160901},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {8},

publisher = {Public Library of Science},

abstract = {Genomic alteration in head and neck squamous cell carcinoma (HNSCC) was studied in two cell line pairs (HN30-HN31 and HN4-HN12) using conventional C-banding, multiplex fluorescence in situ hybridization (M-FISH), and array comparative genomic hybridization (array CGH). HN30 and HN4 were derived from primary lesions in the pharynx and base of tongue, respectively, and HN31 and HN12 were derived from lymph-node metastatic lesions belonging to the same patients. Gain of chromosome 1, 7, and 11 were shared in almost all cell lines. Hierarchical clustering revealed that HN31 was closely related to HN4, which shared eight chromosome alteration cases. Large C-positive heterochromatins were found in the centromeric region of chromosome 9 in HN31 and HN4, which suggests complex structural amplification of the repetitive sequence. Array CGH revealed amplification of 7p22.3p11.2, 8q11.23q12.1, and 14q32.33 in all cell lines involved with tumorigenesis and inflammation genes. The amplification of 2p21 (SIX3), 11p15.5 (H19), and 11q21q22.3 (MAML2, PGR, TRPC6, and MMP family) regions, and deletion of 9p23 (PTPRD) and 16q23.1 (WWOX) regions were identified in HN31 and HN12. Interestingly, partial loss of PTPRD (9p23) and WWOX (16q23.1) genes was identified in HN31 and HN12, and the level of gene expression tended to be the down-regulation of PTPRD, with no detectable expression of the WWOX gene. This suggests that the scarcity of PTPRD and WWOX genes might have played an important role in progression of HNSCC, and could be considered as a target for cancer therapy or a biomarker in molecular pathology. © 2016 Singchat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Suntronpong2016,

title = {CENP-B box, a nucleotide motif involved in centromere formation, occurs in a New World monkey},

author = {A. Suntronpong and K. Kugou and H. Masumoto and K. Srikulnath and K. Ohshima and H. Hirai and A. Koga},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84974700452&doi=10.1098%2frsbl.2015.0817&partnerID=40&md5=9aad3b2b0486e502231763fbfc7074b3},

doi = {10.1098/rsbl.2015.0817},

issn = {17449561},

year  = {2016},

date = {2016-01-01},

journal = {Biology Letters},

volume = {12},

number = {3},

publisher = {Royal Society},

abstract = {Centromere protein B (CENP-B) is one of the major proteins involved in centromere formation, binding to centromeric repetitive DNA by recognizing a 17 bp motif called the CENP-B box. Hominids (humans and great apes) carry large numbers of CENP-B boxes in alpha satellite DNA (AS, the major centromeric repetitive DNA of simian primates). Only negative results have been reported regarding the presence of the CENP-B box in other primate taxa. Consequently, it is widely believed that the CENP-B box is confined, within primates, to the hominids. We report here that the common marmoset, a New World monkey, contains an abundance of CENP-B boxes in its AS. First, in a long contig sequence we constructed and analysed, we identified the motif in 17 of the 38 alpha satellite repeat units. We then sequenced terminal regions of additional clones and found the motif in many of them. Immunostaining of marmoset cells demonstrated that CENP-B binds to DNA in the centromeric regions of chromosomes. Therefore, functional CENP-B boxes are not confined to hominids. Our results indicate that the efficiency of identification of the CENP-B box may depend largely on the sequencing methods used, and that the CENP-B box in centromeric repetitive DNA may be more common than researchers previously thought. © 2016 The Author(s) Published by the Royal Society. All rights reserved.},

note = {cited By 15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sujiwattanarat2016187,

title = {Molecular cloning and characterization of Siamese crocodile (Crocodylus siamensis) copper, zinc superoxide dismutase (CSI-Cu,Zn-SOD) gene},

author = {P. Sujiwattanarat and P. Pongsanarakul and Y. Temsiripong and T. Temsiripong and C. Thawornkuno and Y. Uno and S. Unajak and Y. Matsuda and K. Choowongkomon and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946594160&doi=10.1016%2fj.cbpa.2015.10.028&partnerID=40&md5=4ebc5f9dd84a87234febb5afc2c1b049},

doi = {10.1016/j.cbpa.2015.10.028},

issn = {10956433},

year  = {2016},

date = {2016-01-01},

journal = {Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology},

volume = {191},

pages = {187-195},

publisher = {Elsevier Inc.},

abstract = {Superoxide dismutase (SOD, EC 1.15.1.1) is an antioxidant enzyme found in all living cells. It regulates oxidative stress by breaking down superoxide radicals to oxygen and hydrogen peroxide. A gene coding for Cu,Zn-SOD was cloned and characterized from Siamese crocodile (Crocodylus siamensis; CSI). The full-length expressed sequence tag (EST) of this Cu,Zn-SOD gene (designated as CSI-Cu,Zn-SOD) contained 462bp encoding a protein of 154 amino acids without signal peptides, indicated as intracellular CSI-Cu,Zn-SOD. This agreed with the results from the phylogenetic tree, which indicated that CSI-Cu,Zn-SOD belonged to the intracellular Cu,Zn-SOD. Chromosomal location determined that the CSI-Cu,Zn-SOD was localized to the proximal region of the Siamese crocodile chromosome 1p. Several highly conserved motifs, two conserved signature sequences (GFHVHEFGDNT and GNAGGRLACGVI), and conserved amino acid residues for binding copper and zinc (His47, His49, His64, His72, His81, Asp84, and His120) were also identified in CSI-Cu,Zn-SOD. Real-time PCR analysis showed that CSI-Cu,Zn-SOD mRNA was expressed in all the tissues examined (liver, pancreas, lung, kidney, heart, and whole blood), which suggests a constitutively expressed gene in these tissues. Expression of the gene in Escherichia coli cells followed by purification yielded a recombinant CSI-Cu,Zn-SOD, with Km and Vmax values of 6.075mM xanthine and 1.4×10-3mmolmin-1mg-1, respectively. This Vmax value was 40 times lower than native Cu,Zn-SOD (56×10-3mmolmin-1mg-1), extracted from crocodile erythrocytes. This suggests that cofactors, protein folding properties, or post-translational modifications were lost during the protein purification process, leading to a reduction in the rate of enzyme activity in bacterial expression of CSI-Cu,Zn-SOD. © 2015 Elsevier Inc.},

note = {cited By 19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsubara2015529,

title = {Molecular cloning and characterization of satellite DNA sequences from constitutive heterochromatin of the habu snake (Protobothrops flavoviridis, Viperidae) and the Burmese python (Python bivittatus, Pythonidae)},

author = {K. Matsubara and Y. Uno and K. Srikulnath and R. Seki and C. Nishida and Y. Matsuda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949084964&doi=10.1007%2fs00412-015-0529-6&partnerID=40&md5=bbdde200cc570f935d512b57a483173d},

doi = {10.1007/s00412-015-0529-6},

issn = {00095915},

year  = {2015},

date = {2015-01-01},

journal = {Chromosoma},

volume = {124},

number = {4},

pages = {529-539},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Highly repetitive DNA sequences of the centromeric heterochromatin provide valuable molecular cytogenetic markers for the investigation of genomic compartmentalization in the macrochromosomes and microchromosomes of sauropsids. Here, the relationship between centromeric heterochromatin and karyotype evolution was examined using cloned repetitive DNA sequences from two snake species, the habu snake (Protobothrops flavoviridis, Crotalinae, Viperidae) and Burmese python (Python bivittatus, Pythonidae). Three satellite DNA (stDNA) families were isolated from the heterochromatin of these snakes: 168-bp PFL-MspI from P. flavoviridis and 196-bp PBI-DdeI and 174-bp PBI-MspI from P. bivittatus. The PFL-MspI and PBI-DdeI sequences were localized to the centromeric regions of most chromosomes in the respective species, suggesting that the two sequences were the major components of the centromeric heterochromatin in these organisms. The PBI-MspI sequence was localized to the pericentromeric region of four chromosome pairs. The PFL-MspI and the PBI-DdeI sequences were conserved only in the genome of closely related species, Gloydius blomhoffii (Crotalinae) and Python molurus, respectively, although their locations on the chromosomes were slightly different. In contrast, the PBI-MspI sequence was also in the genomes of P. molurus and Boa constrictor (Boidae), and additionally localized to the centromeric regions of eight chromosome pairs in B. constrictor, suggesting that this sequence originated in the genome of a common ancestor of Pythonidae and Boidae, approximately 86 million years ago. The three stDNA sequences showed no genomic compartmentalization between the macrochromosomes and microchromosomes, suggesting that homogenization of the centromeric and/or pericentromeric stDNA sequences occurred in the macrochromosomes and microchromosomes of these snakes. © 2015, Springer-Verlag Berlin Heidelberg.},

note = {cited By 18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Unajak2015585,

title = {Molecular characterization of Galectin-8 from Nile tilapia (Oreochromis niloticus Linn.) and its response to bacterial infection},

author = {S. Unajak and N. Pholmanee and N. Songtawee and K. Srikulnath and P. Srisapoome and A. Kiataramkul and H. Kondo and I. Hirono and N. Areechon},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84948967283&doi=10.1016%2fj.molimm.2015.09.012&partnerID=40&md5=3a5407b726de846254fa81a669365908},

doi = {10.1016/j.molimm.2015.09.012},

issn = {01615890},

year  = {2015},

date = {2015-01-01},

journal = {Molecular Immunology},

volume = {68},

number = {2},

pages = {585-596},

publisher = {Elsevier Ltd},

abstract = {Galectins belong to the family of galactoside-binding proteins and play a major role in the immune and inflammatory responses of vertebrates and invertebrates. The galectin family is divided into three subtypes based on molecular structure; prototypes, chimera types, and tandem-repeated types. We isolated and characterized the cDNA of galectin-8 (OnGal-8) in Nile tilapia (Oreochromis niloticus). OnGal-8 consisted of a 966 bp open reading frame (ORF) that encoded a 321 amino acid protein (43.47 kDa). Homology and phylogenetic tree analysis suggested the protein was clustered with galectin-8s from other animal species and shared at least 56.8% identity with salmon galectin-8. Structurally, the amino acid sequence included two distinct N- and C- terminus carbohydrate recognition domains (CRDs) of 135 and 133 amino acids, respectively, that were connected by a 39 amino acid polypeptide linker. The N- and C-CRDs contained two conserved WG-E-I and WG-E-T motifs, suggesting they have an important role in mediating the specific interactions between OnGal-8 and saccharide moieties such as β-galactoside. The structure of OnGal-8 was characterized by a two-fold symmetric pattern of 10-and 12-stranded antiparallel ß-sheets of both N- and C-CRDs, and the peptide linker presumably formed a random coil similar to the characteristic tandem-repeat type galectin. The expression of OnGal-8 in healthy fish was highest in the skin, intestine, and brain. Experimental challenge of Nile tilapia with S. agalactiae resulted in significant up-regulation of OnGal-8 in the spleen after 5 d. Our results suggest that OnGal-8 is involved in the immune response to bacterial infection. © 2015 Elsevier Ltd.},

note = {cited By 16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Supikamolseni201513981,

title = {Molecular barcoding of venomous snakes and species-specific multiplex PCR assay to identify snake groups for which antivenom is available in thailand},

author = {A. Supikamolseni and N. Ngaoburanawit and M. Sumontha and L. Chanhome and S. Suntrarachun and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946021489&doi=10.4238%2f2015.October.29.18&partnerID=40&md5=079d87e1d3d49bdef70ff8b5d894efd6},

doi = {10.4238/2015.October.29.18},

issn = {16765680},

year  = {2015},

date = {2015-01-01},

journal = {Genetics and Molecular Research},

volume = {14},

number = {4},

pages = {13981-13997},

publisher = {Fundacao de Pesquisas Cientificas de Ribeirao Preto},

abstract = {DNA barcodes of mitochondrial COI and Cytb genes were constructed from 54 specimens of 16 species for species identification. Intra-and interspecific sequence divergence of the COI gene (10 times) was greater than that of the Cytb gene (4 times), which suggests that the former gene may be a better marker than the latter for species delimitation in snakes. The COI barcode cut-off scores differed by more than 3% between most species, and the minimum interspecific divergence was greater than the maximum intraspecific divergence. Clustering analysis indicated that most species fell into monophyletic clades. These results suggest that these species could be reliably differentiated using COI DNA barcodes. Moreover, a novel species-specific multiplex PCR assay was developed to distinguish between Naja spp, Ophiophagus hannah, Trimeresurus spp, Hydrophiinae, Daboia siamensis, Bungarus fasciatus, and Calloselasma rhodostoma. Antivenom for these species is produced and kept by the Thai Red Cross for clinical use. Our novel PCR assay could easily be applied to venom and saliva samples and could be used effectively for the rapid and accurate identification of species during forensic work, conservation study, and medical research. © FUNPEC-RP.},

note = {cited By 63},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Srikulnath2015,

title = {Karyotype reorganization in the Hokou Gecko (Gekko hokouensis, Gekkonidae): The process of microchromosome disappearance in Gekkota},

author = {K. Srikulnath and Y. Uno and C. Nishida and H. Ota and Y. Matsuda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942163763&doi=10.1371%2fjournal.pone.0134829&partnerID=40&md5=d0e927509f2631b408dda6c129022ca4},

doi = {10.1371/journal.pone.0134829},

issn = {19326203},

year  = {2015},

date = {2015-01-01},

journal = {PLoS ONE},

volume = {10},

number = {8},

publisher = {Public Library of Science},

abstract = {The Hokou gecko (Gekko hokouensis: Gekkonidae, Gekkota, Squamata) has the chromosome number 2n = 38, with no microchromosomes. For molecular cytogenetic characterization of the gekkotan karyotype, we constructed a cytogenetic map for G. hokouensis, which retains the ancestral karyotype of Gekkota, with 86 functional genes, and compared it with cytogenetic maps for four Toxicofera species that have many microchromosomes (Elaphe quadrivirgata, Varanus salvator macromaculatus, Leiolepis reevesii rubritaeniata, and Anolis carolinensis) and that for a lacertid species (Lacerta agilis) with only one pair of autosomal microchromosomes. Ten pairs of G. hokouensis chromosomes [GHO1, 2, 3, Z(4), 6, 7, 8, 13, 14, and 15] showed highly conserved linkage homology with macrochromosomes and/or macrochromosome arms of the four Toxicofera species and corresponded to eight L. agilis macrochromosomes (LAG). However, GHO5, GHO9, GHO10, GHO11, and LAG6 were composed of chromosome segments that have a homology with Toxicofera microchromosomes, and no homology was found in the chromosomes between G. hokouensis and L. agilis. These results suggest that repeated fusions of microchromosomes may have occurred independently in each lineage of Gekkota and Lacertidae, leading to the disappearance of microchromosomes and appearance of small-sized macrochromosomes. © 2015 Srikulnath et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Srikulnath2015243,

title = {Phylogenetic relationship of Dendrobium species in Thailand inferred from chloroplast matK gene and nuclear rDNA ITS region},

author = {K. Srikulnath and S. Sawasdichai and T. K. Jantapanon and P. Pongtongkam and S. Peyachoknagul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937216986&doi=10.2503%2fhortj.MI-028&partnerID=40&md5=d53b9dc5307e95a12ec8b84fd321e96c},

doi = {10.2503/hortj.MI-028},

issn = {21890102},

year  = {2015},

date = {2015-01-01},

journal = {Horticulture Journal},

volume = {84},

number = {3},

pages = {243-252},

publisher = {Japanese Society for Horticultural Science},

abstract = {In Thailand, Dendrobium is the most popular crop grown for cut flowers and as a pot plant for local use and export. Some native species are used for hybrid production while some are specific to particular parts of the country and need to be conserved. The sequences of rDNA ITS (ITS1-5.8S-ITS2) and the matK gene were used to reconstruct the phylogenetic relationships of 27 native Dendrobium spp. in Thailand, which are composed of 9 sections: Breviflores, Callista, Dendrobium, Distichophyllum, Formosae, Pedilonum, Rhopalanthe, Stachyobium, and Strongyle. The average levels of nucleotide divergence (p-distances) of matK and rDNA ITS sequences were 1.3% and 11.2%, respectively. The topologies of the phylogenetic trees reconstructed based on matK and rDNA ITS sequences were slightly different. Use of the rDNA ITS sequence was shown to be a more authoritative systematic approach than use of the matK gene sequence in the genus Dendrobium. The result of the combined data (matK-rDNA ITS) was highly similar to the result from rDNA ITS. From rDNA ITS and combined data, the twenty-seven Dendrobium spp. were clearly grouped into two clades with some differences from the morphological classification. The present study provides the first molecular phylogeny of 27 native Dendrobium species in Thailand. © 2015 The Japanese Society for Horticultural Science (JSHS), All rights reserved.},

note = {cited By 9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sujiwattanarat2015,

title = {Higher-order repeat structure in alpha satellite DNA occurs in New World monkeys and is not confined to hominoids},

author = {P. Sujiwattanarat and W. Thapana and K. Srikulnath and Y. Hirai and H. Hirai and A. Koga},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929346913&doi=10.1038%2fsrep10315&partnerID=40&md5=b11905bfdd9e2b6b031710180068336b},

doi = {10.1038/srep10315},

issn = {20452322},

year  = {2015},

date = {2015-01-01},

journal = {Scientific Reports},

volume = {5},

publisher = {Nature Publishing Group},

abstract = {Centromeres usually contain large amounts of tandem repeat DNA. Alpha satellite DNA (AS) is the most abundant tandem repeat DNA found in the centromeres of simian primates. The AS of humans contains sequences organized into higher-order repeat (HOR) structures, which are tandem arrays of larger repeat units consisting of multiple basic repeat units. HOR-carrying AS also occurs in other hominoids, but results reported to date for phylogenetically more remote taxa have been negative. Here we show direct evidence for clear HOR structures in AS of the owl monkey and common marmoset. These monkeys are New World monkey species that are located phylogenetically outside of hominoids. It is currently postulated that the presence of HOR structures in AS is unique to hominoids. Our results suggest that this view must be modified. A plausible explanation is that generation of HOR structures is a general event that occurs occasionally or frequently in primate centromeres, and that, in humans, HOR-carrying AS became predominant in the central region of the centromere. It is often difficult to assemble sequence reads of tandem repeat DNAs into accurate contig sequences; our careful sequencing strategy allowed us to overcome this problem. © 2015, Nature Publishing Group. All rights reserved.},

note = {cited By 20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Trirongjitmoah2015148,

title = {Classification of garlic cultivars using an electronic nose},

author = {S. Trirongjitmoah and Z. Juengmunkong and K. Srikulnath and P. Somboon},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924024753&doi=10.1016%2fj.compag.2015.02.007&partnerID=40&md5=73a64ffc9c2e37cad984a0d475a44754},

doi = {10.1016/j.compag.2015.02.007},

issn = {01681699},

year  = {2015},

date = {2015-01-01},

journal = {Computers and Electronics in Agriculture},

volume = {113},

pages = {148-153},

publisher = {Elsevier B.V.},

abstract = {We developed an electronic nose method for classifying garlic cultivars. Each garlic cultivar gave different semiconductor gas sensor array response patterns, which we analysed using two-dimensional principal component analysis (PCA). The method was able to detect the differences between cultivars using a PCA-optimized set of five sensors without any significant loss of performance compared to the original eight sensors. The performance of the electronic nose system was confirmed using two alternative methods, namely (1) the cluster analysis of the genetic relationships between garlic cultivars using amplified fragment length polymorphism (AFLP) markers, and (2) measuring the concentrations of sulfur-containing compounds in each garlic cultivar by gas chromatography-mass spectrometry (GC-MS). Four Thai garlic cultivars were consistently characterized and placed in three groups using the AFLP, GC-MS, and electronic nose methods. These results suggest that garlic cultivars can be classified simply and quickly using a low-cost electronic nose system. © 2015 Elsevier B.V.},

note = {cited By 42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsubara2015753,

title = {No interstitial telomeres on autosomes but remarkable amplification of telomeric repeats on the W sex chromosome in the sand lizard (Lacerta agilis)},

author = {K. Matsubara and Y. Uno and K. Srikulnath and Y. Matsuda and E. Miller and M. Olsson},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84959327443&doi=10.1093%2fjhered%2fesv083&partnerID=40&md5=341292d5e4d1582488b24c4c292310be},

doi = {10.1093/jhered/esv083},

issn = {00221503},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Heredity},

volume = {106},

number = {6},

pages = {753-757},

publisher = {Oxford University Press},

abstract = {Telomeres are repeat (TTAGGG)n sequences that form terminal ends of chromosomes and have several functions, such as protecting the coding DNA from erosion at mitosis. Due to chromosomal rearrangements through evolutionary history (e.g., inversions and fusions), telomeric sequences are also found between the centromere and the terminal ends (i.e., at interstitial telomeric sites, ITSs). ITS telomere sequences have been implicated in heritable disease caused by genomic instability of ITS polymorphic variants, both with respect to copy number and sequence. In the sand lizard (Lacerta agilis), we have shown that telomere length is predictive of lifetime fitness in females but not males. To assess whether this sex specific fitness effect could be traced to ITSs differences, we mapped (TTAGGG)n sequences using fluorescence in situ hybridization in fibroblast cells cultured from 4 specimens of known sex. No ITSs could be found on autosomes in either sex. However, females have heterogametic sex chromosomes in sand lizards (ZW},

note = {cited By 32},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Wannapinpong201525,

title = {Molecular cloning and characterization of the CHS gene family in turmeric (Curcuma longa Linn.)},

author = {S. Wannapinpong and K. Srikulnath and A. Thongpan and K. Choowongkomon and S. Peyachoknagul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920770955&doi=10.1007%2fs13562-013-0232-8&partnerID=40&md5=e20a1b9514e96fc6c01af1f6f1e84622},

doi = {10.1007/s13562-013-0232-8},

issn = {09717811},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Plant Biochemistry and Biotechnology},

volume = {24},

number = {1},

pages = {25-33},

publisher = {Springer},

abstract = {Three chalcone synthase (CHS) genes were isolated from Curcuma longa Linn. using TAIL-PCR. ClCHS1 and ClCHS2 were 1,460 and 1,407 bp in length, respectively, containing 1,191 bp open reading frame (ORF) that encodes 396 amino acids, whereas ClCHS3 was 1,394 bp in length containing 1,170 bp ORF that encodes 389 amino acids. The structure of all three genes comprise two exons and one intron which are consistent with the other CHS gene family. Southern blot analysis using a ClCHS conserved fragment revealed the ClCHS genes belong to a gene family. Phylogenetic analysis showed the three putative ClCHS proteins to be closely related to DCS (diketide-CoA synthase) protein, a product of CHS-like gene in C. longa, which condenses malonyl-CoA with feruloyl-CoA or coumaroyl-CoA as the substrate in curcuminoid synthesis. These results suggest that the interaction of substrate and enzyme between the three putative ClCHS proteins and DCS might be highly similar. Homology modeling and docking analysis were consistent, indicating that the same substrate (coumaroyl-CoA) can be used in the putative ClCHS1 and ClCHS2 proteins. However, the putative ClCHS3 protein seems to have seven amino acids deletion in a loop involved in the binding site formation, suggesting that the binding site with coumaroyl-CoA might be altered. © 2013, Society for Plant Biochemistry and Biotechnology.},

note = {cited By 6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Srikulnath2014563,

title = {Identification of the linkage group of the Z sex chromosomes of the sand lizard (Lacerta agilis, Lacertidae) and elucidation of karyotype evolution in lacertid lizards},

author = {K. Srikulnath and K. Matsubara and Y. Uno and C. Nishida and M. Olsson and Y. Matsuda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934273197&doi=10.1007%2fs00412-014-0467-8&partnerID=40&md5=cb7a68bf864e48dfc82940cb26f20de3},

doi = {10.1007/s00412-014-0467-8},

issn = {00095915},

year  = {2014},

date = {2014-01-01},

journal = {Chromosoma},

volume = {123},

number = {6},

pages = {563-575},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {The sand lizard (Lacerta agilis, Lacertidae) has a chromosome number of 2n = 38, with 17 pairs of acrocentric chromosomes, one pair of microchromosomes, a large acrocentric Z chromosome, and a micro-W chromosome. To investigate the process of karyotype evolution in L. agilis, we performed chromosome banding and fluorescent in situ hybridization for gene mapping and constructed a cytogenetic map with 86 functional genes. Chromosome banding revealed that the Z chromosome is the fifth largest chromosome. The cytogenetic map revealed homology of the L. agilis Z chromosome with chicken chromosomes 6 and 9. Comparison of the L. agilis cytogenetic map with those of four Toxicofera species with many microchromosomes (Elaphe quadrivirgata, Varanus salvator macromaculatus, Leiolepis reevesii rubritaeniata, and Anolis carolinensis) showed highly conserved linkage homology of L. agilis chromosomes (LAG) 1, 2, 3, 4, 5(Z), 7, 8, 9, and 10 with macrochromosomes and/or macrochromosome segments of the four Toxicofera species. Most of the genes located on the microchromosomes of Toxicofera were localized to LAG6, small acrocentric chromosomes (LAG11–18), and a microchromosome (LAG19) in L. agilis. These results suggest that the L. agilis karyotype resulted from frequent fusions of microchromosomes, which occurred in the ancestral karyotype of Toxicofera and led to the disappearance of microchromosomes and the appearance of many small macrochromosomes. © 2014, Springer-Verlag Berlin Heidelberg.},

note = {cited By 45},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Baicharoen2014,

title = {Locational diversity of alpha satellite DNA and intergeneric hybridization aspects in the nomascusand hylobatesgenera of small apes},

author = {S. Baicharoen and T. Miyabe-Nishiwaki and V. Arsaithamkul and Y. Hirai and K. Duangsa-Ard and B. Siriaroonrat and H. Domae and K. Srikulnath and A. Koga and H. Hirai},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907833524&doi=10.1371%2fjournal.pone.0109151&partnerID=40&md5=8583c312118d80bc2cd56e279a09da73},

doi = {10.1371/journal.pone.0109151},

issn = {19326203},

year  = {2014},

date = {2014-01-01},

journal = {PLoS ONE},

volume = {9},

number = {10},

publisher = {Public Library of Science},

abstract = {Recently, we discovered that alpha satellite DNA has unique and genus-specific localizations on the chromosomes of small apes. This study describes the details of alpha satellite localization in the genera Nomascus and Hylobates and explores their usefulness in distinguishing parental genome sets in hybrids between these genera. Fluorescence in situ hybridization was used to establish diagnostic criteria of alpha satellite DNA markers in discriminating small ape genomes. In particular we established the genus specificity of alpha satellite distribution in three species of light-cheeked gibbons (Nomascus leucogenys, N. siki, and N. gabriellae) in comparison to that of Hylobates lar. Then we determined the localization of alpha satellite DNA in a hybrid individual which resulted from a cross between these two genera. In Nomascus the alpha satellite DNA blocks were located at the centromere, telomere, and four interstitial regions. In Hylobates detectable amounts of alpha satellite DNA were seen only at centromeric regions. The differences in alpha satellite DNA locations between Nomascus and Hylobates allowed us to easily distinguish the parental chromosomal sets in the genome of intergeneric hybrid individuals found in Thai and Japanese zoos. Our study illustrates how molecular cytogenetic markers can serve as diagnostic tools to identify the origin of individuals. These molecular tools can aid zoos, captive breeding programs and conservation efforts in managing small apes species. Discovering more information on alpha satellite distribution is also an opportunity to examine phylogenetic and evolutionary questions that are still controversial in small apes. © 2014 Baicharoen et al.},

note = {cited By 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chailertrit20147157,

title = {Isolation and characterization of novel microsatellite markers from Siamese fighting fish (Betta splendens, Osphronemidae, Anabantoidei) and their transferability to related species, B. smaragdina and B. imbellis},

author = {V. Chailertrit and A. Swatdipong and S. Peyachoknagul and J. Salaenoi and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907152027&doi=10.4238%2f2014.September.5.1&partnerID=40&md5=22cae53b1b21078038446261994084d8},

doi = {10.4238/2014.September.5.1},

issn = {16765680},

year  = {2014},

date = {2014-01-01},

journal = {Genetics and Molecular Research},

volume = {13},

number = {3},

pages = {7157-7162},

publisher = {Fundacao de Pesquisas Cientificas de Ribeirao Preto},

abstract = {Ten novel microsatellite markers were developed and characterized from Siamese fighting fish (Betta splendens). Nine of ten markers were polymorphic, exhibiting an allelic number (NA) from 2 to 6 alleles per locus. The effective number of alleles (NE) ranged from 1.60 to 3.08 (average of 2.30). The observed (HO) and expected (HE) heterozygosities ranged from 0.13 to 0.67 (average of 0.39) and 0.29 to 0.63 (average of 0.50), respectively. Linkage disequilibrium was not significantly detected for any pair of loci, and only two loci (BettaMS23 and BettaMS28) showed significant deviations from Hardy-Weinberg expectations. Of these, six loci could be amplified in genomic DNA of the closely related species B. imbellis and three loci in B. smaragdina. These microsatellite markers could be used as a tool to investigate genetic diversity and population structure, as well as breeding programs in hatcheries. © FUNPEC-RP.},

note = {cited By 12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Peyachoknagul20145441,

title = {Development of microsatellite markers of vandaceous orchids for species and variety identification},

author = {S. Peyachoknagul and C. Nettuwakul and P. Phuekvilai and S. Wannapinpong and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904981683&doi=10.4238%2f2014.July.24.23&partnerID=40&md5=e56f4327f2830c1a491020792805ba50},

doi = {10.4238/2014.July.24.23},

issn = {16765680},

year  = {2014},

date = {2014-01-01},

journal = {Genetics and Molecular Research},

volume = {13},

number = {3},

pages = {5441-5445},

publisher = {Fundacao de Pesquisas Cientificas de Ribeirao Preto},

abstract = {Vandaceous orchids are a group of orchid genera in the subfamily Vandoideae. Among this group, Mokara, Phalaenopsis, and Vanda are the most popular and commercially important orchids in Thailand. Novel microsatellite markers were developed from Mokara, the intergeneric hybrid from 3 genera Vanda, Ascocentrum, and Arachnis by using enriched method. Six primers from this study plus one primer previously developed from Vanda genome, a total of 7 markers, were selected to characterize 4 orchid genera (Mokara, Vanda, Rhynchostylis, and Ascocenda). The observed and expected heterozygosities varied in the 4 genera from 0.0000-1.0000 and 0.0000-0.8765, respectively. The transferability of these primers was also investigated in 76 vandaceous orchids from 12 genera. Three primer pairs, MOK26, MOK29, and MOK62, could successfully amplify the DNA of all samples, while MOK103 could be used with most of the samples. The total number of alleles from 76 samples ranged from 3 to 19 alleles per locus, with an average of 8.5714. Therefore, these markers could be used for variety/ species identification, certification and protection, genetic diversity, and evolutionary studies. © FUNPEC-RP.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Thapana2014,

title = {Reduction in the structural instability of cloned eukaryotic tandem-repeat DNA by low-temperature culturing of host bacteria},

author = {W. Thapana and P. Sujiwattanarat and K. Srikulnath and H. Hirai and A. Koga},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929325494&doi=10.1017%2fS0016672314000172&partnerID=40&md5=7bf3a9b51618ab592ab7685bd861529c},

doi = {10.1017/S0016672314000172},

issn = {00166723},

year  = {2014},

date = {2014-01-01},

journal = {Genetics Research},

volume = {96},

publisher = {Cambridge University Press},

abstract = {For accurate analyses of eukaryotic tandem-repeat DNA, it is often required to clone a genomic DNA fragment into a bacterial plasmid. It is, however, a serious problem that tandem-repeat DNA is frequently subjected to structural changes during maintenance or amplification in the host bacteria. Here, we show an example of a clear difference in the instability of tandem-repeat DNA between different culturing temperatures. A fragment of monkey centromeric DNA carried by pUC19 was considerably degraded by culturing bacteria at 37 °C, but the damage was reduced at 25 °C. Thus, culturing temperature is a significant factor for avoiding degradation, in addition to the genotype of the host bacteria. © 2014 Cambridge University Press.},

note = {cited By 7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}