@article{Majtánová20201,

title = {Evidence of interspecific chromosomal diversification in rainbowfishes (Melanotaeniidae, teleostei)},

author = {Z. Majtánová and P. J. Unmack and T. Prasongmaneerut and F. Shams and K. Srikulnath and P. Ráb and T. Ezaz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088164284&doi=10.3390%2fgenes11070818&partnerID=40&md5=ec376baf2ec99647ab7d924e5a75f26a},

doi = {10.3390/genes11070818},

issn = {20734425},

year  = {2020},

date = {2020-01-01},

journal = {Genes},

volume = {11},

number = {7},

pages = {1-11},

publisher = {MDPI AG},

abstract = {Rainbowfishes (Melanotaeniidae) are the largest monophyletic group of freshwater fishes occurring in Australia and New Guinea, with 112 species currently recognised. Despite their high taxonomic diversity, rainbowfishes remain poorly studied from a cytogenetic perspective. Using conventional (Giemsa staining, C banding, chromomycin A3 staining) and molecular (fluorescence in situ hybridisation with ribosomal DNA (rDNA) and telomeric probes) cytogenetic protocols, karyotypes and associated chromosomal characteristics of five species were examined. We covered all major lineages of this group, namely, Running River rainbowfish Melanotaenia sp., red rainbowfish Glossolepis incisus, threadfin rainbowfish Iriatherina werneri, ornate rainbowfish Rhadinocentrus ornatus, and Cairns rainbowfish Cairnsichthys rhombosomoides. All species had conserved diploid chromosome numbers 2n = 48, but karyotypes differed among species; while Melanotaenia sp., G. incisus, and I. werneri possessed karyotypes composed of exclusively subtelo/acrocentric chromosomes, the karyotype of R. ornatus displayed six pairs of submetacentric and 18 pairs of subtelo/acrocentric chromosomes, while C. rhombosomoides possessed a karyotype composed of four pairs of submetacentric and 20 pairs of subtelo/acrocentric chromosomes. No heteromorphic sex chromosomes were detected using conventional cytogenetic techniques. Our data indicate a conserved 2n in Melanotaeniidae, but morphologically variable karyotypes, rDNA sites, and heterochromatin distributions. Differences were observed especially in taxonomically divergent species, suggesting interspecies chromosome rearrangements. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {cited By 3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Alam20201,

title = {Cross-species bac mapping highlights conservation of chromosome synteny across dragon lizards (Squamata: Agamidae)},

author = {S. M. I. Alam and M. Altmanová and T. Prasongmaneerut and A. Georges and S. D. Sarre and S. V. Nielsen and T. Gamble and K. Srikulnath and M. Rovatsos and L. Kratochvíl and T. Ezaz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087127134&doi=10.3390%2fgenes11060698&partnerID=40&md5=63d77fab6fefd15f7c14499ac9e9d80e},

doi = {10.3390/genes11060698},

issn = {20734425},

year  = {2020},

date = {2020-01-01},

journal = {Genes},

volume = {11},

number = {6},

pages = {1-12},

publisher = {MDPI AG},

abstract = {Dragon lizards (Squamata: Agamidae) comprise about 520 species in six subfamilies distributed across Asia, Australasia and Africa. Only five species are known to have sex chromosomes. All of them possess ZZ/ZW sex chromosomes, which are microchromosomes in four species from the subfamily Amphibolurinae, but much larger in Phrynocephalus vlangalii from the subfamily Agaminae. In most previous studies of these sex chromosomes, the focus has been on Australian species from the subfamily Amphibolurinae, but only the sex chromosomes of the Australian central bearded dragon (Pogona vitticeps) are well-characterized cytogenetically. To determine the level of synteny of the sex chromosomes of P. vitticeps across agamid subfamilies, we performed cross-species two-colour FISH using two bacterial artificial chromosome (BAC) clones from the pseudo-autosomal regions of P. vitticeps. We mapped these two BACs across representative species from all six subfamilies as well as two species of chameleons, the sister group to agamids. We found that one of these BAC sequences is conserved in macrochromosomes and the other in microchromosomes across the agamid lineages. However, within the Amphibolurinae, there is evidence of multiple chromosomal rearrangements with one of the BACs mapping to the second-largest chromosome pair and to the microchromosomes in multiple species including the sex chromosomes of P. vitticeps. Intriguingly, no hybridization signal was observed in chameleons for either of these BACs, suggesting a likely agamid origin of these sequences. Our study shows lineage-specific evolution of sequences/syntenic blocks and successive rearrangements and reveals a complex history of sequences leading to their association with important biological processes such as the evolution of sex chromosomes and sex determination. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {cited By 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jangtarwan2020,

title = {Predictive genetic plan for a captive population of the Chinese goral (Naemorhedus griseus) and prescriptive action for ex situ and in situ conservation management in Thailand},

author = {K. Jangtarwan and P. Kamsongkram and N. Subpayakom and S. Sillapaprayoon and N. Muangmai and A. Kongphoemph and A. Wongsodchuen and S. Intapan and W. Chamchumroon and M. Safoowong and S. Peyachoknagul and P. Duengkae and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086008290&doi=10.1371%2fjournal.pone.0234064&partnerID=40&md5=0dde1bf4591b921bfe07b92887ec5faa},

doi = {10.1371/journal.pone.0234064},

issn = {19326203},

year  = {2020},

date = {2020-01-01},

journal = {PLoS ONE},

volume = {15},

number = {6},

publisher = {Public Library of Science},

abstract = {Captive breeding programs for endangered species can increase population numbers for eventual reintroduction to the wild. Captive populations are typically small and isolated, which results in inbreeding and reduction of genetic variability, and may lead to an increased risk of extinction. The Omkoi Wildlife Breeding Center maintains the only Thai captive Chinese goral (Naemorhedus griseus) population, and has plans to reintroduce individuals into natural isolated populations. Genetic variability was assessed within the captive population using microsatellite data. Although no bottleneck was observed, genetic variability was low (allelic richness = 7.091 ± 0.756},

note = {cited By 11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Singchat2020209,

title = {Do sex chromosomes of snakes, monitor lizards, and iguanian lizards result from multiple fission of an “ancestral amniote super-sex chromosome”?},

author = {W. Singchat and S. Sillapaprayoon and N. Muangmai and S. Baicharoen and C. Indananda and P. Duengkae and S. Peyachoknagul and R. E. O’Connor and D. K. Griffin and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085105523&doi=10.1007%2fs10577-020-09631-4&partnerID=40&md5=4a270a6da3994c8b654465da3920a8a5},

doi = {10.1007/s10577-020-09631-4},

issn = {09673849},

year  = {2020},

date = {2020-01-01},

journal = {Chromosome Research},

volume = {28},

number = {2},

pages = {209-228},

publisher = {Springer},

abstract = {Sex chromosomes in some amniotes share linkage homologies with distantly related taxa in regions orthologous to squamate reptile chromosome 2 (SR2) and the snake W sex chromosome. Thus, the SR2 and W chromosomes may formerly have been part of a larger ancestral amniote super-sex chromosome. Comparison of various sex chromosomal linkage homologies in Toxicofera with those in other amniotes offers an excellent model to assess key cytological differences, to understand the mechanisms of amniote sex chromosome evolution in each lineage and the existence of an ancestral amniote super-sex chromosome. Chromosome maps of four species of Toxicofera were constructed using bacterial artificial chromosomes (BACs) derived from chicken and zebra finch libraries containing amniote sex chromosomal linkages. Different macrochromosome linkage homologies were highly conserved among Toxicofera, and at least two BACs (CH261-125F1 and CH261-40D6) showed partial homology with sex chromosomes of amniotes associated with SR2, which supports the hypothesis of an ancestral super-sex chromosome with overlaps of partial linkage homologies. The present data also suggest a possible multiple fission mechanism of an ancestral super-sex chromosome, which resulted in further development of various sex chromosomal linkages of Toxicofera based on particular properties that favored the role of sex chromosomes. © 2020, Springer Nature B.V.},

note = {cited By 15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ahmad20203856,

title = {Next-generation sequencing yields complete mitochondrial genome assembly of peaceful betta fish, Betta imbellis (Teleostei: Osphronemidae)},

author = {S. F. Ahmad and N. Laopichienpong and W. Singchat and A. Suntronpong and T. Pongsanarm and T. Panthum and N. Ariyaraphong and J. Bulan and T. Pansrikaew and K. Jangtarwan and N. Subpayakom and S. Dokkaew and N. Muangmai and P. Duengkae and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097991831&doi=10.1080%2f23802359.2020.1841582&partnerID=40&md5=06b7011c7216ed2aa20e9e54c2ec2e82},

doi = {10.1080/23802359.2020.1841582},

issn = {23802359},

year  = {2020},

date = {2020-01-01},

journal = {Mitochondrial DNA Part B: Resources},

volume = {5},

number = {4},

pages = {3856-3858},

publisher = {Taylor and Francis Ltd.},

abstract = {The complete mitochondrial genome (mitogenome) of the peaceful betta (Betta imbellis) was obtained using next-generation sequencing. The sample of B. imbellis was collected from its native habitat in Southern Thailand. The mitogenome sequence was 16,897 bp in length, containing 37 genes with identical order to most teleost mitogenomes. Overall nucleotide base composition of the complete mitogenome was determined as AT bias. Phylogenetic analysis of B. imbellis showed a closer relationship with bubble-nesting fighting fish. This annotated mitogenome reference can be utilized as a bioresource for phylogenetic studies to support betta conservation programs. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {cited By 5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Thongchum2019,

title = {Diversity of PBI-DdeI satellite DNA in snakes correlates with rapid independent evolution and different functional roles},

author = {R. Thongchum and W. Singchat and N. Laopichienpong and P. Tawichasri and E. Kraichak and O. Prakhongcheep and S. Sillapaprayoon and N. Muangmai and S. Baicharoen and S. Suntrarachun and L. Chanhome and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074289724&doi=10.1038%2fs41598-019-51863-w&partnerID=40&md5=817072a72cbff4b31f29de8868a2382b},

doi = {10.1038/s41598-019-51863-w},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {To better understand PBI-DdeI satellite DNA located in the centromeric region of python, molecular evolution analysis was conducted on 40 snake species. A ladder-like pattern of DNA bands with repetition of the 194–210 bp monomer was observed in 15 species using PCR. Molecular cloning was performed to obtain 97 AT-rich monomer sequences. Phylogenetic and network analyses showed three PBI-DdeI subfamilies with sequences grouped in species-specific clusters, suggesting rapid evolution. Slow evolution was found in eight species with shared PBI-DdeI sequences, suggesting recent species diversification, allowing PBI-DdeI no time to diverge, with limited homogenization and fixation processes. Quantitative real-time PCR showed large differences in copy number between Python bivittatus and other snakes, consistent with repeat scanning of whole genome sequences. Copy numbers were significantly higher in female Naja kaouthia than in males, concurring with chromosomal distribution of PBI-DdeI specifically localized to female W chromosomes. PBI-DdeI might act as an evolutionary driver with several repeats to promote W chromosome differentiation and heterochromatinization in N. kaouthia. Analysis revealed PBI-DdeI with a reduced copy number, compared to P. bivittatus, in most snakes studied, and it is possible that it subsequently dispersed and amplified on W chromosomes with different functional roles in N. kaouthia. © 2019, The Author(s).},

note = {cited By 11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsubara2019,

title = {ZW sex chromosomes in Australian dragon lizards (Agamidae) originated from a combination of duplication and translocation in the nucleolar organising region},

author = {K. Matsubara and D. O’Meally and S. D. Sarre and A. Georges and K. Srikulnath and T. Ezaz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074428025&doi=10.3390%2fgenes10110861&partnerID=40&md5=59644ab7dcb20a11afa78b871315b0a8},

doi = {10.3390/genes10110861},

issn = {20734425},

year  = {2019},

date = {2019-01-01},

journal = {Genes},

volume = {10},

number = {11},

publisher = {MDPI AG},

abstract = {Sex chromosomes in some reptiles share synteny with distantly related amniotes in regions orthologous to squamate chromosome 2. The latter finding suggests that chromosome 2 was formerly part of a larger ancestral (amniote) super-sex chromosome and raises questions about how sex chromosomes are formed and modified in reptiles. Australian dragon lizards (Agamidae) are emerging as an excellent model for studying these processes. In particular, they exhibit both genotypic (GSD) and temperature-dependent (TSD) sex determination, show evidence of transitions between the two modes and have evolved non-homologous ZW sex microchromosomes even within the same evolutionary lineage. They therefore represent an excellent group to probe further the idea of a shared ancestral super-sex chromosome and to investigate mechanisms for transition between different sex chromosome forms. Here, we compare sex chromosome homology among eight dragon lizard species from five genera to identify key cytological differences and the mechanisms that may be driving sex chromosome evolution in this group. We performed fluorescence in situ hybridisation to physically map bacterial artificial chromosome (BAC) clones from the bearded dragon, Pogona vitticeps’ ZW sex chromosomes and a nucleolar organising region (NOR) probe in males and females of eight Agamid species exhibiting either GSD or TSD.We show that the sex chromosome derived BAC clone hybridises near the telomere of chromosome 2q in all eight species examined. This clone also hybridises to the sex microchromosomes of three species (P vitticeps, P. barbata and Diporiphora nobbi) and a pair of microchromosomes in three others (Ctenophorus pictus, Amphibolurus norrisi and Amphibolurus muricatus). No other chromosomes are marked by the probe in two species from the closely related genus Physignathus. A probe bearing nucleolar organising region (NOR) sequences maps close to the telomere of chromosome 2q in all eight species, and to the ZW pair in P. vitticeps and P. barbata, the W microchromosome in D. nobbi, and several microchromosomes in P. cocincinus. Our findings provide evidence of sequence homology between chromosome 2 and the sex chromosomes of multiple agamids. These data support the hypothesis that there was an ancestral sex chromosome in amniotes that gave rise to squamate chromosome 2 and raises the prospect that some particular property of this chromosome has favoured its role as a sex chromosome in amniotes. It is likely that the amplification of repetitive sequences associated with this region has driven the high level of heterochromatinisation of the sex-specific chromosomes in three species of agamid. Our data suggest a possible mechanism for chromosome rearrangement, including inversion and duplication near the telomeric regions of the ancestral chromosome 2 and subsequent translocation to the ZW sex microchromosomes in three agamid species. It is plausible that these chromosome rearrangements involving sex chromosomes also drove speciation in this group. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {cited By 12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jangtarwan2019,

title = {Take one step backward to move forward: Assessment of genetic diversity and population structure of captive Asian woollynecked storks (Ciconia episcopus)},

author = {K. Jangtarwan and T. Koomgun and T. Prasongmaneerut and R. Thongchum and W. Singchat and P. Tawichasri and T. Fukayama and S. Sillapaprayoon and E. Kraichak and N. Muangmai and S. Baicharoen and C. Punkong and S. Peyachoknagul and P. Duengkae and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073095794&doi=10.1371%2fjournal.pone.0223726&partnerID=40&md5=e5b7cb35536deae69d51ad826eb4e8ea},

doi = {10.1371/journal.pone.0223726},

issn = {19326203},

year  = {2019},

date = {2019-01-01},

journal = {PLoS ONE},

volume = {14},

number = {10},

publisher = {Public Library of Science},

abstract = {The fragmentation of habitats and hunting have impacted the Asian woolly-necked stork (Ciconia episcopus), leading to a serious risk of extinction in Thailand. Programs of active captive breeding, together with careful genetic monitoring, can play an important role in facilitating the creation of source populations with genetic variability to aid the recovery of endangered species. Here, the genetic diversity and population structure of 86 Asian woollynecked storks from three captive breeding programs [Khao Kheow Open Zoo (KKOZ) comprising 68 individuals, Nakhon Ratchasima Zoo (NRZ) comprising 16 individuals, and Dusit Zoo (DSZ) comprising 2 individuals] were analyzed using 13 microsatellite loci, to aid effective conservation management. Inbreeding and an extremely low effective population size (Ne) were found in the KKOZ population, suggesting that deleterious genetic issues had resulted from multiple generations held in captivity. By contrast, a recent demographic bottleneck was observed in the population at NRZ, where the ratio of Ne to abundance (N) was greater than 1. Clustering analysis also showed that one subdivision of the KKOZ population shared allelic variability with the NRZ population. This suggests that genetic drift, with a possible recent and mixed origin, occurred in the initial NRZ population, indicating historical transfer between captivities. These captive stork populations require improved genetic variability and a greater population size, which could be achieved by choosing low-related individuals for future transfers to increase the adaptive potential of reintroduced populations. Forward-in-time simulations such as those described herein constitute the first step in establishing an appropriate source population using a scientifically managed perspective for an in situ and ex situ conservation program in Thailand. © 2019 Jangtarwan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Deakin2019,

title = {Chromosomics: Bridging the Gap between Genomes and Chromosomes},

author = {J. E. Deakin and S. Potter and R. O'Neill and A. Ruiz-Herrera and M. B. Cioffi and M. D. B. Eldridge and K. Fukui and J. A. Marshall Graves and D. Griffin and F. Grutzner and L. Kratochvíl and I. Miura and M. Rovatsos and K. Srikulnath and E. Wapstra and T. Ezaz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074258994&doi=10.3390%2fgenes10080627&partnerID=40&md5=32e15604d2f6c6b2d0db3cce6628447c},

doi = {10.3390/genes10080627},

issn = {20734425},

year  = {2019},

date = {2019-01-01},

journal = {Genes},

volume = {10},

number = {8},

publisher = {NLM (Medline)},

abstract = {The recent advances in DNA sequencing technology are enabling a rapid increase in the number of genomes being sequenced. However, many fundamental questions in genome biology remain unanswered, because sequence data alone is unable to provide insight into how the genome is organised into chromosomes, the position and interaction of those chromosomes in the cell, and how chromosomes and their interactions with each other change in response to environmental stimuli or over time. The intimate relationship between DNA sequence and chromosome structure and function highlights the need to integrate genomic and cytogenetic data to more comprehensively understand the role genome architecture plays in genome plasticity. We propose adoption of the term 'chromosomics' as an approach encompassing genome sequencing, cytogenetics and cell biology, and present examples of where chromosomics has already led to novel discoveries, such as the sex-determining gene in eutherian mammals. More importantly, we look to the future and the questions that could be answered as we enter into the chromosomics revolution, such as the role of chromosome rearrangements in speciation and the role more rapidly evolving regions of the genome, like centromeres, play in genome plasticity. However, for chromosomics to reach its full potential, we need to address several challenges, particularly the training of a new generation of cytogeneticists, and the commitment to a closer union among the research areas of genomics, cytogenetics, cell biology and bioinformatics. Overcoming these challenges will lead to ground-breaking discoveries in understanding genome evolution and function.},

note = {cited By 57},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Singchat20196366,

title = {Dynamics of telomere length in captive Siamese cobra (Naja kaouthia) related to age and sex},

author = {W. Singchat and E. Kraichak and P. Tawichasri and T. Tawan and A. Suntronpong and S. Sillapaprayoon and R. Phatcharakullawarawat and N. Muangmai and S. Suntrarachun and S. Baicharoen and V. Punyapornwithaya and S. Peyachoknagul and L. Chanhome and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067395063&doi=10.1002%2fece3.5208&partnerID=40&md5=1df553c474dc381a57ac2d19ad1ef4b2},

doi = {10.1002/ece3.5208},

issn = {20457758},

year  = {2019},

date = {2019-01-01},

journal = {Ecology and Evolution},

volume = {9},

number = {11},

pages = {6366-6377},

publisher = {John Wiley and Sons Ltd},

abstract = {Telomeres comprise tandem repeated DNA sequences that protect the ends of chromosomes from deterioration or fusion with neighboring chromosomes, and their lengths might vary with sex and age. Here, age- and sex-related telomere lengths in male and female captive Siamese cobras (Naja kaouthia) were investigated using quantitative real-time polymerase chain reaction based on cross-sectional data. A negative correlation was shown between telomere length and body size in males but not in females. Age-related sex differences were also recorded. Juvenile female snakes have shorter telomeres relative to males at up to 5 years of age, while body size also rapidly increases during this period. This suggests that an accelerated increase in telomere length of female cobra results from sex hormone stimulation to telomerase activity, reflecting sexually dimorphic phenotypic traits. This might also result from amplification of telomeric repeats on sex chromosomes. By contrast, female Siamese cobras older than 5 years had longer telomeres than males. Diverse sex hormone levels and oxidative stress parameters between sexes may affect telomere length. © 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.},

note = {cited By 7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ponjarat201984,

title = {Evidence of dramatic sterility in F 1 male hybrid catfish [male Clarias gariepinus (Burchell, 1822) × female C. macrocephalus (Günther, 1864)] resulting from the failure of homologous chromosome pairing in meiosis I},

author = {J. Ponjarat and W. Singchat and P. Monkheang and A. Suntronpong and P. Tawichasri and S. Sillapaprayoon and S. Ogawa and N. Muangmai and S. Baicharoen and S. Peyachoknagul and I. Parhar and U. Na-Nakorn and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061960886&doi=10.1016%2fj.aquaculture.2019.02.035&partnerID=40&md5=5ab9240fea3cbea60089f27b9903a101},

doi = {10.1016/j.aquaculture.2019.02.035},

issn = {00448486},

year  = {2019},

date = {2019-01-01},

journal = {Aquaculture},

volume = {505},

pages = {84-91},

publisher = {Elsevier B.V.},

abstract = {Sterile hybrid animals exhibit spermatogenic disruptions with a decreased number and/or malformation of mature sperm. F 1 hybrid catfish (2n = 55) is an important cultured fish derived from male North African catfish [Clarias gariepinus (Burchell, 1822); 2n = 56] and female bighead catfish [C. macrocephalus (Günther, 1864); 2n = 54]; they are sterile with gametogenic failure in males. Despite the generality of this phenomenon, the spermatogenic phenotype has not been well described and a comprehensive understanding of the genetic basis of the disruption remains elusive. Our observations in the F 1 male hybrid showed abnormal morphology of testes with small size, while meiotic configuration indicated that meiosis succeeded in the early pachytene stage but failed to progress beyond the diplotene-diakinesis stage in primary spermatocytes. This suggests the presence of a number of degenerated spermatocytes. Histological examination recorded no postmeiotic cells and spermatozoa in F 1 male hybrid testes. A high frequency of apoptotic testicular cells was also present in the F 1 male hybrid, as shown by histochemical results for activated Caspase-3 and TUNEL assays. Low expression levels of Caspase-3, p53 and MCL1 (isoform 2), which activate pro-apoptotic signals in the development process, were also observed in both parental species, but significantly high expression levels were observed in the F 1 male hybrid. By contrast, the level of BCL2 expression was very low in the F 1 male hybrid, supporting the state of apoptosis as BCL2 inhibits the actions of pro-apoptotic proteins. These results collectively suggest that sterility in male hybrids is caused by spermatogenic disruptions in the pachytene stage, resulting from the failure of homologous chromosome pairing due to chromosomal incompatibility between parental genomes, and subsequent elimination by apoptosis leading to spermatogenic breakdown in the F 1 male hybrid. © 2019 Elsevier B.V.},

note = {cited By 9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Srikulnath2019,

title = {Distribution and amplification of interstitial telomeric sequences (ITSs) in Australian dragon lizards support frequent chromosome fusions in Iguania},

author = {K. Srikulnath and B. Azad and W. Singchat and T. Ezaz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061969083&doi=10.1371%2fjournal.pone.0212683&partnerID=40&md5=7c4f9feefcf9fa8d5092b5fa5789ab8b},

doi = {10.1371/journal.pone.0212683},

issn = {19326203},

year  = {2019},

date = {2019-01-01},

journal = {PLoS ONE},

volume = {14},

number = {2},

publisher = {Public Library of Science},

abstract = {Telomeric sequences are generally located at the ends of chromosomes; however, they can also be found in non-terminal chromosomal regions when they are known as interstitial telomeric sequences (ITSs). Distribution of ITSs across closely related and divergent species elucidates karyotype evolution and speciation as ITSs provide evolutionary evidence for chromosome fusion. In this study, we performed physical mapping of telomeric repeats by fluorescence in situ hybridisation (FISH) in seven Australian dragon lizards thought to represent derived karyotypes of squamate reptiles and a gecko lizard with considerably different karyotypic feature. Telomeric repeats were present at both ends of all chromosomes in all species, while varying numbers of ITSs were also found on microchromosomes and in pericentromeric or centromeric regions on macrochromosomes in five lizard species examined. This suggests that chromosomal rearrangements from ancestral squamate reptiles to Iguania occurred mainly by fusion between ancestral types of acrocentric chromosomes and/or between microchromosomes, leading to appearance of bi-armed macrochromosomes, and in the reduction of microchromosome numbers. These results support the previously proposed hypothesis of karyotype evolution in squamate reptiles. In addition, we observed the presence of telomeric sequences in the similar regions to heterochromatin of the W microchromosome in Pogona barbata and Doporiphora nobbi, while sex chromosomes for the two species contained part of the nucleolar organiser regions (NORs). This likely implies that these ITSs are a part of the satellite DNA and not relics of chromosome fusions. Amplification of telomeric repeats may have involved heterochromatinisation of sex-specific W chromosomes and play a role in the organisation of the nucleolus. © 2019 Srikulnath et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ponjarat2019672,

title = {Complete mitochondrial genome of two mouthbrooding fighting fishes, Betta apollon and B. simplex (Teleostei: Osphronemidae)},

author = {J. Ponjarat and P. Areesirisuk and O. Prakhongcheep and S. Dokkaew and S. Sillapaprayoon and N. Muangmai and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061103038&doi=10.1080%2f23802359.2019.1572463&partnerID=40&md5=94a9f8edb90de5e1505a3538b12031ae},

doi = {10.1080/23802359.2019.1572463},

issn = {23802359},

year  = {2019},

date = {2019-01-01},

journal = {Mitochondrial DNA Part B: Resources},

volume = {4},

number = {1},

pages = {672-674},

publisher = {Taylor and Francis Ltd.},

abstract = {Mouthbrooding fighting fish Betta apollon and B. simplex are widely distributed in Southeast Asia but urbanization is restricting their biodiversity. Complete mitochondrial genomes (mitogenomes) of B. apollon and B. simplex were determined to support systematic conservation programs. Mitogenome sequences were 16,536 and 16,549 bp in length with slight AT bias (56.68% and 56.60%), respectively, containing 37 genes with the order identical to most teleost mitogenomes. Phylogenetic analysis of B. apollon showed a closer relationship with B. simplex, grouped with B. pi as a monophyletic clade of mouthbrooders. Results will facilitate evolutionary studies, species diversity, and conservation management in fighting fishes. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {cited By 6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Singchat2019577,

title = {Complete mitochondrial genome of Siamese cobra (Naja kaouthia) determined using next-generation sequencing},

author = {W. Singchat and P. Areesirisuk and S. Sillapaprayoon and N. Muangmai and S. Baicharoen and S. Suntrarachun and L. Chanhome and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060335258&doi=10.1080%2f23802359.2018.1558123&partnerID=40&md5=3b3eedbd3755c0ff1d2cbad3128d4ef9},

doi = {10.1080/23802359.2018.1558123},

issn = {23802359},

year  = {2019},

date = {2019-01-01},

journal = {Mitochondrial DNA Part B: Resources},

volume = {4},

number = {1},

pages = {577-578},

publisher = {Taylor and Francis Ltd.},

abstract = {Siamese cobra (Naja kaouthia) exhibits highly toxic venom, which causes morbidity and mortality. Accurate species identification through molecular approaches is very important to administer correct antivenoms. The Siamese cobra mitogenome contains 17,203 bp with slight AT bias (58.2%) containing 37 genes in identical order to snake mitogenomes; no tandem repeat was found in the control region. Phylogenetic analysis indicated that Siamese and other cobras had highly supported monophyletic clades similar to the genus Naja and close relationships with other elapid snakes. Our results will facilitate clinical diagnosis and enrich genomic resources for future evolutionary studies and conservation management. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {cited By 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ThongtamNaAyudhaya2019222,

title = {Complete mitochondrial genome of 10 anemonefishes belonging to Amphiprion and Premnas},

author = {P. Thongtam Na Ayudhaya and P. Areesirisuk and W. Singchat and S. Sillapaprayoon and N. Muangmai and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059771540&doi=10.1080%2f23802359.2018.1546145&partnerID=40&md5=f916bfbc3997ac10314075c97eaf689b},

doi = {10.1080/23802359.2018.1546145},

issn = {23802359},

year  = {2019},

date = {2019-01-01},

journal = {Mitochondrial DNA Part B: Resources},

volume = {4},

number = {1},

pages = {222-224},

publisher = {Taylor and Francis Ltd.},

abstract = {Anemonefish are widely distributed in tropical areas with phenotypic color variation often observed in the same species. Complete mitochondrial genomes (mitogenomes) of 10 anemonefishes belonging to Amphiprion and Premnas were determined to support taxonomic status. Average mitogenome sequence was 16,838 ± 19.69 bp, containing 37 genes with identical gene order to most teleost mitogenomes. The percula complex comprised A. percula and A. ocellaris and was phylogenetically clustered with P. biaculeatus. Color morphs of A. ocellaris and P. biaculeatus were identified, suggesting large phenotypic variation at species level. Results will facilitate further genetic studies of mitochondrial variation and species diversity in anemonefish. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Thongchum2019301,

title = {The CENP-B box, a nucleotide motif involved in centromere formation, has multiple origins in new world monkeys},

author = {R. Thongchum and H. Nishihara and K. Srikulnath and H. Hirai and A. Koga},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078692463&doi=10.1266%2fggs.19-00042&partnerID=40&md5=bf18145b8a1d59b91645f3e45c3341b8},

doi = {10.1266/ggs.19-00042},

issn = {13417568},

year  = {2019},

date = {2019-01-01},

journal = {Genes and Genetic Systems},

volume = {94},

number = {6},

pages = {301-306},

publisher = {Genetics Society of Japan},

abstract = {Centromere protein B (CENP-B), a protein participating in centromere formation, binds to centromere satellite DNA by recognizing a 17-bp motif called the CENP-B box. This motif is found in hominids (humans and great apes) at an identical location in repeat units of their centromere satellite DNA. We have recently reported that the CENP-B box exists at diverse locations in three New World monkey species (marmoset, squirrel monkey and tamarin). However, the evolutionary origin of the CENP-B box in these species was not determined. It could have been present in a common ancestor, or emerged multiple times in different lineages. Here we present results of a phylogenetic analysis of centromere satellite DNA that support the multiple emergence hypothesis. Repeat units almost invariably formed monophyletic groups in each species and the CENP-B box location was unique for each species. The CENP-B box is not essential for the immediate survival of its host organism. On the other hand, it is known to be required for de novo centromere assembly. Our results suggest that the CENP-B box confers a long-term selective advantage. For example, it may play a pivotal role when a centromere is accidentally lost or impaired. © 2019, Genetics Society of Japan. All rights reserved.},

note = {cited By 6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Singchat2018,

title = {Chromosome map of the Siamese cobra: Did partial synteny of sex chromosomes in the amniote represent "a hypothetical ancestral super-sex chromosome" or random distribution?},

author = {W. Singchat and R. E. O'Connor and P. Tawichasri and A. Suntronpong and S. Sillapaprayoon and S. Suntrarachun and N. Muangmai and S. Baicharoen and S. Peyachoknagul and L. Chanhome and D. Griffin and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058664294&doi=10.1186%2fs12864-018-5293-6&partnerID=40&md5=1b10cc139b88cc5df0da192abbd95c78},

doi = {10.1186/s12864-018-5293-6},

issn = {14712164},

year  = {2018},

date = {2018-01-01},

journal = {BMC Genomics},

volume = {19},

number = {1},

publisher = {BioMed Central},

abstract = {Background: Unlike the chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid chromosome number of 38 with a highly heterochromatic W chromosome and a large morphologically different chromosome 2. To investigate the process of sex chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a chromosome map of the Siamese cobra (Naja kaouthia) with 43 bacterial artificial chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. Results: We produced a detailed chromosome map of the Siamese cobra genome, focusing on chromosome 2 and sex chromosomes. Synteny of the Siamese cobra chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)n and six microsatellite repeat motifs ((AAGG)8, (AGAT)8, (AAAC)8, (ACAG)8, (AATC)8, and (AAAAT)6) were observed on NKAW, and most of these were also found on other amniote sex chromosomes. Conclusions: The frequent amplification of repeats might involve heterochromatinization and promote sex chromosome differentiation in the Siamese cobra W sex chromosome. Repeat sequences are also shared among amniote sex chromosomes, which supports the hypothesis of an ancestral super-sex chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex chromosomes. © 2018 The Author(s).},

note = {cited By 31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Boonkaew2018735,

title = {Characterization of GA20ox genes in tall and dwarf types coconut (Cocos nucifera L.)},

author = {T. Boonkaew and C. Mongkolsiriwatana and A. Vongvanrungruang and K. Srikulnath and S. Peyachoknagul},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044345047&doi=10.1007%2fs13258-018-0682-4&partnerID=40&md5=136af7bcfa75351238fddf58d3a23e8d},

doi = {10.1007/s13258-018-0682-4},

issn = {19769571},

year  = {2018},

date = {2018-01-01},

journal = {Genes and Genomics},

volume = {40},

number = {7},

pages = {735-745},

publisher = {Genetics Society of Korea},

abstract = {Coconuts (Cocos nucifera L.) are divided by the height into tall and dwarf types. In many plants the short phenotype was emerged by mutation of the GA20ox gene encoding the enzyme involved in gibberellin (GA) biosynthesis. Two CnGA20ox genes, CnGA20ox1 and CnGA20ox2, were cloned from tall and dwarf types coconut. The sequences, gene structures and expressions were compared. The structure of each gene comprised three exons and two introns. The CnGA20ox1 and CnGA20ox2 genes consisted of the coding region of 1110 and 1131 bp, encoding proteins of 369 and 376 amino acids, respectively. Their amino acid sequences are highly homologous to GA20ox1 and GA20ox2 genes of Elaeis guineensis, but only 57% homologous to each other. However, the characteristic amino acids two histidines and one aspartic acid which are the two iron (Fe2+) binding residues, and arginine and serine which are the substrate binding residues of the dioxygenase enzyme in the 20G-FeII_Oxy domain involved in GA biosynthesis, were found in the active site of both enzymes. The evolutionary relationship of their proteins revealed three clusters in vascular plants, with two subgroups in dicots and three subgroups in monocots. This result confirmed that CnGA20ox was present as multi-copy genes, and at least two groups CnGA20ox1 and CnGA20ox2 were found in coconut. The nucleotide sequences of CnGA20ox1 gene in both coconut types were identical but its expression was about three folds higher in the leaves of tall coconut than in those of dwarf type which was in good agreement with their height. In contrast, the nucleotide sequences of CnGA20ox2 gene in the two coconut types were different, but the expression of CnGA20ox2 gene could not be detected in either coconut type. The promoter region of CnGA20ox1 gene was cloned, and the core promoter sequences and various cis-elements were found. The CnGA20ox1 gene should be responsible for the height in coconut, which is different from other plants because no mutation was present in CnGA20ox1 gene of dwarf type coconut. © 2018, The Genetics Society of Korea and Springer Science+Business Media B.V., part of Springer Nature.},

note = {cited By 13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Prakhongcheep20186,

title = {Complete mitochondrial genome of mouthbrooding fighting fish (Betta pi) compared with bubble nesting fighting fish (B. splendens)},

author = {O. Prakhongcheep and N. Muangmai and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037725259&doi=10.1080%2f23802359.2017.1413294&partnerID=40&md5=fff951c482e46d2a68d968143156abfd},

doi = {10.1080/23802359.2017.1413294},

issn = {23802359},

year  = {2018},

date = {2018-01-01},

journal = {Mitochondrial DNA Part B: Resources},

volume = {3},

number = {1},

pages = {6-8},

publisher = {Taylor and Francis Ltd.},

abstract = {Betta pi is the largest species of mouthbrooding fighting fish, while B. splendens is a globally ornamental bubble nesting fish. Complete mitochondrial genomes (mitogenomes) of wild individuals of B. pi and B. splendens were determined. The mitogenome sequences were 16,521 and 16,980 base pair in length, containing 37 genes with gene order identical to most teleost mitogenomes. Overall A + T content was 57.72% for B. pi and 61.92% for B. splendens. Phylogenetic analysis showed that B. pi and B. splendens were highly supported monophyletic clades. Our results will facilitate further genetic studies, including mitochondrial variations and population structure of fighting fishes. © 2018, © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {cited By 14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lisachov20181083,

title = {New karyotype of Lygosoma bowringii suggests cryptic diversity},

author = {A. Lisachov and N. Poyarkov and P. Pawangkhanant and P. Borodin and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060281279&partnerID=40&md5=6c8c27780b8d219406435b54866dace5},

issn = {20715773},

year  = {2018},

date = {2018-01-01},

journal = {Herpetology Notes},

volume = {11},

pages = {1083-1088},

publisher = {Societas Europaea Herpetologica},

abstract = {In the family Scincidae, karyotypes of most species have diploid numbers of 28–32 chromosomes. They are bimodal, i.e. there are two distinct size classes of chromosomes: microchromosomes and macrochromosomes. Previous karyotypic studies of the common supple skink, Lygosoma bowringii, showed the karyotype comprising 18 macro-and 14 microchromosomes (2n=32), which is common for the family. Here, we described a new derived unimodal karyotype of L. bowringii with 2n=24, observed in individuals from Bangkok (Thailand). A difference between the newly and previously described karyotypes of this species suggests the possibility of cryptic intraspecific chromosomal diversity. © 2018, Societas Europaea Herpetologica. All rights reserved.},

note = {cited By 2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Areesirisuk2018,

title = {Characterization of five complete Cyrtodactylus mitogenome structures reveals low structural diversity and conservation of repeated sequences in the lineage},

author = {P. Areesirisuk and N. Muangmai and K. Kunya and W. Singchat and S. Sillapaprayoon and S. Lapbenjakul and W. Thapana and A. Kantachumpoo and S. Baicharoen and B. Rerkamnuaychoke and S. Peyachoknagul and K. Han and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059200773&doi=10.7717%2fpeerj.6121&partnerID=40&md5=87e65663040a0a8b52904fbc1a2dd149},

doi = {10.7717/peerj.6121},

issn = {21678359},

year  = {2018},

date = {2018-01-01},

journal = {PeerJ},

volume = {2018},

number = {12},

publisher = {PeerJ Inc.},

abstract = {Mitochondrial genomes (mitogenomes) of five Cyrtodactylus were determined. Their compositions and structures were similar to most of the available gecko lizard mitogenomes as 13 protein-coding, two rRNA and 22 tRNA genes. The non-coding control region (CR) of almost all Cyrtodactylus mitogenome structures contained a repeated sequence named the 75-bp box family, except for C. auribalteatus which contained the 225-bp box. Sequence similarities indicated that the 225-bp box resulted from the duplication event of 75-bp boxes, followed by homogenization and fixation in C. auribalteatus. The 75-bp box family was found in most gecko lizards with high conservation (55-75% similarities) and could form secondary structures, suggesting that this repeated sequence family played an important role under selective pressure and might involve mitogenome replication and the likelihood of rearrangements in CR. The 75-bp box family was acquired in the common ancestral genome of the gecko lizard, evolving gradually through each lineage by independent nucleotide mutation. Comparison of gecko lizard mitogenomes revealed low structural diversity with at least six types of mitochondrial gene rearrangements. Cyrtodactylus mitogenome structure showed the same gene rearrangement as found in most gecko lizards. Advanced mitogenome information will enable a better understanding of structure evolution mechanisms. © Copyright 2018 Areesirisuk et al.},

note = {cited By 4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tawichasri20176,

title = {Using blood and non-invasive shed skin samples to identify sex of caenophidian snakes based on multiplex PCR assay},

author = {P. Tawichasri and N. Laopichienpong and L. Chanhome and R. Phatcharakullawarawat and W. Singchat and T. Koomgun and T. Prasongmaneerut and W. Rerkamnuaychoke and S. Sillapaprayoon and N. Muangmai and S. Suntrarachun and S. Baicharoen and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85033465736&doi=10.1016%2fj.jcz.2017.11.003&partnerID=40&md5=5aa747f8655dbd6546e671dcc62aa504},

doi = {10.1016/j.jcz.2017.11.003},

issn = {00445231},

year  = {2017},

date = {2017-01-01},

journal = {Zoologischer Anzeiger},

volume = {271},

pages = {6-14},

publisher = {Elsevier GmbH},

abstract = {Molecular sexing is routinely used in the fields of forensic investigations, population genetics and conservation biology. However, none of the assays used so far allows non-ambiguous, quick, and cheap sex identification in snakes. Here, we designed a new multiplex PCR (two panels of triplex) sexing assay using two homologous loci on sex chromosomes (snake gametologous genes), CTNNB1Z/CTNNB1W and WACZ/WACW, and two combined female-specific CTNNB1W loci. This method was successfully tested on 81 samples from 17 caenophidian snakes, but not for 20 samples from 5 henophidian snakes, suggesting that this assay was applicable across all caenophidian snake tested. Moreover, the results of the validation study comprising sensitivity, specificity, blind test, and casework with blood spots on filter paper and shed skin as a non-invasive sample confirmed the effectiveness of this assay in the forensic perspective. The assay represents a robust, cheap, rapid, and simple method which should also prove useful in unknown bodies of carcass. This efficient assay should benefit wildlife forensic laboratories and registered breeders to conform to wildlife regulations or certification of commercial trade, and boost profits for snake-breeders and conservationist snake-breeding communities. © 2017 Elsevier GmbH},

note = {cited By 10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Twilprawat20171129,

title = {Structural variations generated by simian foamy virus-like (SFV) in Crocodylus siamensis},

author = {P. Twilprawat and S. Kim and K. Srikulnath and K. Han},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020538781&doi=10.1007%2fs13258-017-0581-0&partnerID=40&md5=092aca72b7c97dfd7128dc513602fbda},

doi = {10.1007/s13258-017-0581-0},

issn = {19769571},

year  = {2017},

date = {2017-01-01},

journal = {Genes and Genomics},

volume = {39},

number = {10},

pages = {1129-1138},

publisher = {Genetics Society of Korea},

abstract = {Endogenous retrovirus (ERV) integrates into the germline of its host and could remain in the genome as a molecular fossil. ERV is one of sources that cause INDEL and recombination events in the vertebrate genomes, leading to various genomic and genetic changes in their hosts. There have been many studies conducted on ERVs in the vertebrate genomes to elucidate their evolutionary history. However, ERVs have not been studied well in Crocodylus siamensis. Here, we report structural variations among SFV1 elements (simian foamy virus-like), ERVs in C. siamensis. We initially identified 26 SFV1 candidates in the genome and experimentally verified 9 SFV1_1 and 5 SFV1_10 elements using PCR display. Their structural analyses showed that most of them are solitary-LTRs but two SFV1_1 elements are full-length. Through further analyses, we found that the two full-length elements retain intact ORFs. We examined transcription factor binding sites within their LTR sequences to predict promoter/enhancer activities. In sum, we identified 14 crocodile-specific SFV1 elements and the results of their structural analyses suggest that they could contribute to genomic or phenotypic variations in C. siamensis population. © 2017, The Genetics Society of Korea and Springer Science+Business Media B.V.},

note = {cited By 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lapbenjakul2017,

title = {High genetic diversity and demographic history of captive Siamese and Saltwater crocodiles suggest the first step toward the establishment of a breeding and reintroduction program in Thailand},

author = {S. Lapbenjakul and W. Thapana and P. Twilprawat and N. Muangmai and T. Kanchanaketu and Y. Temsiripong and S. Unajak and S. Peyachoknagul and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029909175&doi=10.1371%2fjournal.pone.0184526&partnerID=40&md5=670b49dde012039ba9d9ab786e370d44},

doi = {10.1371/journal.pone.0184526},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {9},

publisher = {Public Library of Science},

abstract = {The Siamese crocodile (Crocodylus siamensis) and Saltwater crocodile (C. porosus) are two of the most endangered animals in Thailand. Their numbers have been reduced severely by hunting and habitat fragmentation. A reintroduction plan involving captive-bred populations that are used commercially is important and necessary as a conservation strategy to aid in the recovery of wild populations. Here, the genetic diversity and population structure of 69 individual crocodiles, mostly members of captive populations, were analyzed using both mitochondrial D-loop DNA and microsatellite markers. The overall haplotype diversity was 0.924–0.971 and the mean expected heterozygosity across 22 microsatellite loci was 0.578–0.701 for the two species. This agreed with the star-like shaped topology of the haplotype network, which suggests a high level of genetic diversity. The mean ratio of the number of alleles to the allelic range (M ratio) for the populations of both species was considerably lower than the threshold of 0.68, which was interpreted as indicative of a historical genetic bottleneck. Microsatellite markers provided evidence of introgression for three individual crocodiles, which suggest that hybridization might have occurred between C. siamensis and C. porosus. D-loop sequence analysis detected bi-directional hybridization between male and female individuals of the parent species. Therefore, identification of genetically non-hybrid and hybrid individuals is important for long-term conservation management. Relatedness values were low within the captive populations, which supported their genetic integrity and the viability of a breeding and reintroduction management plan. This work constitutes the first step in establishing an appropriate source population from a scientifically managed perspective for an in situ/ex situ conservation program and reintroduction of crocodile individuals to the wild in Thailand. © 2017 Lapbenjakul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {cited By 22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Prakhongcheep2017,

title = {Lack of satellite DNA species-specific homogenization and relationship to chromosomal rearrangements in monitor lizards (Varanidae, Squamata)},

author = {O. Prakhongcheep and W. Thapana and A. Suntronpong and W. Singchat and K. Pattanatanang and R. Phatcharakullawarawat and N. Muangmai and S. Peyachoknagul and K. Matsubara and T. Ezaz and K. Srikulnath},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027499572&doi=10.1186%2fs12862-017-1044-6&partnerID=40&md5=f46bca8048e78cc1baa4c098f2cb8dd6},

doi = {10.1186/s12862-017-1044-6},

issn = {14712148},

year  = {2017},

date = {2017-01-01},

journal = {BMC Evolutionary Biology},

volume = {17},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Satellite DNAs (stDNAs) are highly repeated sequences that constitute large portions of any genome. The evolutionary dynamics of stDNA (e.g. copy number, nucleotide sequence, location) can, therefore, provide an insight into genome organization and evolution. We investigated the evolutionary origin of VSAREP stDNA in 17 monitor lizards (seven Asian, five Australian, and five African) at molecular and cytogenetic level. Results: Results revealed that VSAREP is conserved in the genome of Asian and Australian varanids, but not in African varanids, suggesting that these sequences are either differentiated or lost in the African varanids. Phylogenetic and arrangement network analyses revealed the existence of at least four VSAREP subfamilies. The similarity of each sequence unit within the same VSAREP subfamily from different species was higher than those of other VSAREP subfamilies belonging to the same species. Additionally, all VSAREP subfamilies isolated from the three Australian species (Varanus rosenbergi, V. gouldii, and V. acanthurus) were co-localized near the centromeric or pericentromeric regions of the macrochromosomes, except for chromosomes 3 and 4 in each Australian varanid. However, their chromosomal arrangements were different among species. Conclusions: The VSAREP stDNA family lack homogenized species-specific nucleotide positions in varanid lineage. Most VSAREP sequences were shared among varanids within the four VSAREP subfamilies. This suggests that nucleotide substitutions in each varanid species accumulated more slowly than homogenization rates in each VSAREP subfamily, resulting in non-species-specific evolution of stDNA profiles. Moreover, changes in location of VSAREP stDNA in each Australian varanid suggests a correlation with chromosomal rearrangements, leading to karyotypic differences among these species. © 2017 The Author(s).},

note = {cited By 10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}